Document Detail


Simple 1H NMR spectroscopic method for assay of salts of the contrast agent diatrizoate in commercial solutions.
MedLine Citation:
PMID:  8757438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, accurate, and specific 1H NMR spectroscopic method was developed for the assay of diatrizoate meglumine or the combination diatrizoate meglumine and diatrizoate sodium in commercial solutions for injection. A mixture of injectable solution and sodium acetate, the internal standard, was diluted with D2O and the 1H NMR spectrum of the solution was obtained. Two approaches were used to calculate the drug content, based on the integral values for the -N-CO-CH3 protons of diatrizoic acid at 2.23 ppm, and -N-CH3 protons of meglumine at 2.73 ppm, and the CH3-CO-protons of sodium acetate at 1.9 ppm. Recoveries (mean +/- standard deviation) of diatrizoic acid and meglumine from 10 synthetic mixtures of various amounts of these compounds with a fixed amount of internal standard were 100.3 +/- 0.55% and 100.1 +/- 0.98%, respectively. In addition to providing a direct means of simultaneously assaying diatrizoic acid and meglumine, the proposed NMR method can also be used to identify diatrizoate meglumine and each of its molecular components.
Authors:
G M Hanna; C A Lau-Cam
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  79     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1996 Jul-Aug
Date Detail:
Created Date:  1997-02-14     Completed Date:  1997-02-14     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
Affiliation:
US Food and Drug Administration, Northeast Regional Laboratory, Brooklyn, NY 11232, USA.
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MeSH Terms
Descriptor/Qualifier:
Contrast Media / analysis*
Diatrizoate / analysis*,  chemistry
Magnetic Resonance Spectroscopy
Meglumine / analysis*,  chemistry
Reference Standards
Solutions
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Solutions; 117-96-4/Diatrizoate; 6284-40-8/Meglumine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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