| Similarity in the metabolic profile in macroscopically involved and un-involved colonic mucosa in patients with inflammatory bowel disease: an in vitro proton ((1)H) MR spectroscopy study. | |
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MedLine Citation:
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PMID: 20418044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The histological extent of inflammatory bowel disease (IBD) is greater than that evident by colonoscopic evaluation. We hypothesized that metabolic profile in macroscopically un-involved colonic mucosa in IBD is similar to that of controls with healthy colon. We thus assessed the differences in metabolic profile in macroscopically involved and un-involved colonic mucosa of IBD patients to further substantiate the extent of disease. PATIENTS AND METHODS: Colonic mucosal biopsies were obtained and snap frozen from both the macroscopically un-involved and involved colonic mucosa of IBD patients and macroscopically normal colonic mucosa of controls and were subjected to in-vitro high-resolution proton ((1)H) magnetic resonance (MR) spectroscopy and the concentrations of metabolites were determined. RESULTS: Thirty-two metabolites were assigned in the proton MR spectrum of colonic mucosa of IBD patients. The concentrations of amino acids (isoleucine, leucine, valine, arginine, lysine, glutamine/glutamate, alanine), membrane metabolites (choline, glycerophosphorylcholine/phosphorylcholine), glycolytic product (lactate) and short chain fatty acid (formate) were significantly lower while significantly high level of glucose were observed in the macroscopically un-involved colonic mucosa of IBD patients compared to the macroscopically normal mucosa of controls. There was no significant difference in the concentrations of metabolites in macroscopically involved and un-involved colonic mucosa of IBD patients. CONCLUSIONS: The metabolic profile in macroscopically un-involved colonic mucosa of IBD patients is similar to that of macroscopically involved mucosa but different from colonic mucosa of controls. This suggests that even macroscopically un-involved colonic mucosa is metabolically abnormal and may explain the increase in extent of disease with time. |
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Authors:
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Uma Sharma; Rajiv R Singh; Vineet Ahuja; Govind K Makharia; Naranamangalam R Jagannathan |
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Publication Detail:
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Type: Journal Article Date: 2010-04-24 |
Journal Detail:
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Title: Magnetic resonance imaging Volume: 28 ISSN: 1873-5894 ISO Abbreviation: Magn Reson Imaging Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2011-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8214883 Medline TA: Magn Reson Imaging Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1022-9 Citation Subset: IM |
Affiliation:
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Department of NMR and MRI Facility, All India Institute of Medical Sciences, New Delhi, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biological Markers / metabolism* Biopolymers / metabolism* Colon / metabolism* Humans Inflammatory Bowel Diseases / metabolism* Intestinal Mucosa / metabolism* Magnetic Resonance Spectroscopy / methods* Protons / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Biopolymers; 0/Protons |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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