| Similarity and Divergence of Phylogenies, Antimicrobial Susceptibilities and Virulence Factor Profiles of Escherichia coli Isolates Causing Persistence and Reinfection of Recurrent Urinary Tract Infections. | |
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MedLine Citation:
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PMID: 23035197 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In order to obtain a better molecular understanding of recurrent urinary tract infection (RUTI), we collected 75 cases with repeatedly occurring uncomplicated UTI. The genetic relationships among uropathogenic Escherichia coli (UPEC) isolates were analyzed by pulsed field gel electrophoresis. While 39 (52%) of the RUTI were defined as persistence with the same strain as the primary infecting strain, 36 (48%) were characterized by reinfection with a new strain different from the primary strain. We then examined the antimicrobial susceptibilities and phylogenetic background of 39 persistences and 86 reinfections UPEC isolates, and screened 44 virulence factor (VF) genes. We found the following items with a significant difference: phylogenetic group B2 (41% vs. 21%; P = 0.0193), adhesin genes: iha (49% vs. 28%; P = 0.0233); papG allele I' (51% vs. 24%; P = 0.003); iron-uptake genes: fyuA (85% vs. 58%; P = 0.0037); irp-2 (87% vs. 65%; P = 0.0109); iutA (87. % vs. 58%; P = 0.0014); and aggregate VF score (median, 11 vs. 9: P = 0.0030). In addition, 41% of persistence strains harbored three adhesin genes simultaneously, whereas it was 22% (P = 0.0289) for reinfection isolates. Moreover, it was 59% vs. 29.% (P = 0.0014) for persistence and reinfection isolates containing seven types of iron-uptake genes. Taken together, the antimicrobial susceptibilities of UPEC isolates had little effect on the RUTI. Compared with reinfection strains, persistence UPEC isolates exhibited higher VF scores and carried more VF genes that may be involved in the development and progression of RUTI. |
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Authors:
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Yanping Luo; Yanning Ma; Qiang Zhao; Leili Wang; Ling Guo; Liyan Ye; Youjiang Zhang; Jiyong Yang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: - ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Microbiology, Chinese PLA General Hospital, Beijing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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