Document Detail


Similarity and divergence of phylogenies, antimicrobial susceptibilities, and virulence factor profiles of Escherichia coli isolates causing recurrent urinary tract infections that persist or result from reinfection.
MedLine Citation:
PMID:  23035197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to obtain a better molecular understanding of recurrent urinary tract infection (RUTI), we collected 75 cases with repeatedly occurring uncomplicated UTI. The genetic relationships among uropathogenic Escherichia coli (UPEC) isolates were analyzed by pulsed-field gel electrophoresis. While 39 (52%) of the RUTI cases were defined as "persistence" of the same strain as the primary infecting strain, 36 (48%) were characterized by "reinfection" with a new strain that is different from the primary strain. We then examined the antimicrobial susceptibilities and phylogenetic backgrounds of 39 persistence and 86 reinfection UPEC isolates, and screened 44 virulence factor (VF) genes. We found that isolates had significant differences in the following: placement in phylogenetic group B2 (41% versus 21%; P = 0.0193) and the presence of adhesin genes iha (49% versus 28%; P = 0.0233) and papG allele I' (51% versus 24%; P = 0.003), iron uptake genes fyuA (85% versus 58%; P = 0.0037), irp-2 (87% versus 65%; P = 0.0109), and iutA (87% versus 58%; P = 0.0014), and an aggregate VF score (median, 11 versus 9; P = 0.0030). In addition, 41% of persistence strains harbored three adhesin genes simultaneously, whereas 22% of reinfection isolates did (P = 0.0289). Moreover, 59% versus 29% (P = 0.0014) of persistence and reinfection isolates contained seven types of iron uptake genes. Taken together, the antimicrobial susceptibilities of UPEC isolates had little effect on the RUTI. Compared with reinfection strains, persistence UPEC isolates exhibited higher VF scores and carried more VF genes than may be involved in the development and progression of RUTI.
Authors:
Yanping Luo; Yanning Ma; Qiang Zhao; Leili Wang; Ling Guo; Liyan Ye; Youjiang Zhang; Jiyong Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-10-24    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4002-7     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Chinese PLA General Hospital, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Aged
Anti-Bacterial Agents / pharmacology*
Electrophoresis, Gel, Pulsed-Field
Escherichia coli / classification*,  drug effects,  genetics,  isolation & purification*
Escherichia coli Infections / microbiology*
Female
Genetic Variation*
Humans
Male
Microbial Sensitivity Tests
Molecular Typing
Phylogeny
Recurrence
Urinary Tract Infections / microbiology*
Virulence Factors / genetics*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Virulence Factors
Comments/Corrections
Comment In:
J Urol. 2013 Oct;190(4):1253   [PMID:  24029323 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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