Document Detail

Similarities and differences between the E5 oncoproteins of bovine papillomaviruses type 1 and type 4: cytoskeleton, motility and invasiveness in E5-transformed bovine and mouse cells.
MedLine Citation:
PMID:  16168512     Owner:  NLM     Status:  MEDLINE    
Bovine papillomaviruses (BPVs) are oncogenic viruses. In cattle, BPV-1/2 is associated with urinary bladder cancer and BPV-4 with upper GI tract cancer. BPV E5 is a small hydrophobic protein localised in the endoplasmic reticulum (ER) and Golgi apparatus (GA). E5 is the major transforming protein of BPVs, capable of inducing cell transformation in cultured mouse fibroblasts and, in cooperation with E7, in primary bovine cells. E5-induced cell transformation is accompanied by activation of several cellular protein kinases, including growth factor receptors, and alkalinisation of endosomes and GA. We have reported that BPV E5 causes swelling and fragmentation of the GA and extensive vacuolisation of the cytoplasm. We now show that E5 from both BPV-1 and BPV-4 disturbs the actin cytoskeleton and focal adhesions in transformed bovine cells, where these morphological and behavioural characteristics are accompanied by hyperphosphorylation of the cellular phosphotyrosine kinase c-src. Both BPV-1 and BPV-4 E5 increase the motility of transformed mouse cells, but only BPV-1 E5 causes transformed mouse cells to penetrate a matrigel matrix. BPV-1 transformed mouse cells, but not BPV-4 transformed mouse cells, have hyperhpsphorylated c-src.
Emmanuella Tsirimonaki; Robina Ullah; Barbara Marchetti; G Hossein Ashrafi; Lynn McGarry; Brad Ozanne; M Saveria Campo
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-15
Journal Detail:
Title:  Virus research     Volume:  115     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-17     Completed Date:  2006-05-16     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  158-68     Citation Subset:  IM    
Institute of Comparative Medicine, University of Glasgow, Garscube Estate, Glasgow G61 1QH, Scotland, UK.
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MeSH Terms
Actins / metabolism
Bovine papillomavirus 1 / physiology*
Bovine papillomavirus 4
Cell Line
Cell Movement*
Cell Transformation, Viral*
Cytoskeleton / ultrastructure*
Focal Adhesions / ultrastructure*
NIH 3T3 Cells
Oncogene Proteins, Viral / physiology*
Protein-Tyrosine Kinases / metabolism
Reg. No./Substance:
0/Actins; 0/Oncogene Proteins, Viral; 0/oncogene protein E5, Bovine papillomavirus type 1; EC Kinases; EC tyrosine-protein kinase

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