| Similarities and differences between the E5 oncoproteins of bovine papillomaviruses type 1 and type 4: cytoskeleton, motility and invasiveness in E5-transformed bovine and mouse cells. | |
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MedLine Citation:
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PMID: 16168512 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bovine papillomaviruses (BPVs) are oncogenic viruses. In cattle, BPV-1/2 is associated with urinary bladder cancer and BPV-4 with upper GI tract cancer. BPV E5 is a small hydrophobic protein localised in the endoplasmic reticulum (ER) and Golgi apparatus (GA). E5 is the major transforming protein of BPVs, capable of inducing cell transformation in cultured mouse fibroblasts and, in cooperation with E7, in primary bovine cells. E5-induced cell transformation is accompanied by activation of several cellular protein kinases, including growth factor receptors, and alkalinisation of endosomes and GA. We have reported that BPV E5 causes swelling and fragmentation of the GA and extensive vacuolisation of the cytoplasm. We now show that E5 from both BPV-1 and BPV-4 disturbs the actin cytoskeleton and focal adhesions in transformed bovine cells, where these morphological and behavioural characteristics are accompanied by hyperphosphorylation of the cellular phosphotyrosine kinase c-src. Both BPV-1 and BPV-4 E5 increase the motility of transformed mouse cells, but only BPV-1 E5 causes transformed mouse cells to penetrate a matrigel matrix. BPV-1 transformed mouse cells, but not BPV-4 transformed mouse cells, have hyperhpsphorylated c-src. |
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Authors:
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Emmanuella Tsirimonaki; Robina Ullah; Barbara Marchetti; G Hossein Ashrafi; Lynn McGarry; Brad Ozanne; M Saveria Campo |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-15 |
Journal Detail:
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Title: Virus research Volume: 115 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-17 Completed Date: 2006-05-16 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 158-68 Citation Subset: IM |
Affiliation:
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Institute of Comparative Medicine, University of Glasgow, Garscube Estate, Glasgow G61 1QH, Scotland, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Bovine papillomavirus 1 / physiology* Bovine papillomavirus 4 Cattle Cell Line Cell Movement* Cell Transformation, Viral* Cytoskeleton / ultrastructure* Focal Adhesions / ultrastructure* Mice NIH 3T3 Cells Oncogene Proteins, Viral / physiology* Phosphorylation Protein-Tyrosine Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Oncogene Proteins, Viral; 0/oncogene protein E5, Bovine papillomavirus type 1; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/CSK tyrosine-protein kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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