Document Detail


Similar proteomic profiles of human mesenchymal stromal cells from different donors.
MedLine Citation:
PMID:  19333800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AIMS: Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in different aspects of hematopoiesis, and seem to modulate the immune system. MSC appear to be promising candidates for cellular therapy associated with BM transplantation (BMT). METHODS: We compared protein expression profiles of MSC cultures derived from different BM donors using two-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) tandem mass spectrometry (MS/MS), and compared mixed lymphocyte reaction (MLR) assays in the absence and presence of third-party human (h) MSC derived from different donors during the same culture passage. RESULTS: In a window of observation (pH 4-7, molecular weight 10-220 kDa), about 172 protein spots were obtained in each 2-D gel, corresponding to 84 distinct proteins. A comparative analysis demonstrated a very similar proteomic profile of cells of the first passage derived from different donors, suggesting that these cells have the same expression pattern. Additionally, cells derived from different donors were equally able to inhibit lymphocyte proliferation. CONCLUSIONS: These results encourage the use of third-party MSC in cellular therapies, as cells derived from different individuals seem to have the same proteomic pattern and exhibit functionally similar properties.
Authors:
Carolina Lazzarotto-Silva; Renata Binato; Bárbara Du Rocher; Júlia Assunção Costa E Costa; Luciana Pizzatti; Luis Fernando Bouzas; Eliana Abdelhay
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cytotherapy     Volume:  11     ISSN:  1477-2566     ISO Abbreviation:  Cytotherapy     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-29     Completed Date:  2009-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100895309     Medline TA:  Cytotherapy     Country:  England    
Other Details:
Languages:  eng     Pagination:  268-77     Citation Subset:  IM    
Affiliation:
Bone Marrow Transplantation Unit, National Cancer Institute, Rio de Janeiro, Brazil. clazza13@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis
Cells, Cultured
Dexamethasone / metabolism
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling
Humans
Immunosuppression
Lymphocyte Culture Test, Mixed
Mesenchymal Stem Cells / cytology,  immunology,  metabolism*
Osteogenesis
Proteome*
Stromal Cells / cytology,  immunology,  metabolism*
T-Lymphocytes / cytology,  immunology,  metabolism*
Tandem Mass Spectrometry
Tissue Donors*
Chemical
Reg. No./Substance:
0/Proteome; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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