| Similar potency of catechin and its enantiomers in alleviating 1-methyl-4-phenylpyridinium ion cytotoxicity in SH-SY5Y cells. | |
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MedLine Citation:
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PMID: 21827489 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objectives Previously, the flavonoid (±)-catechin was shown to exert potent neuroprotective action in the mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model. The purpose of this study was to investigate whether the different enantiomers of catechin ((+)-catechin, (-)-catechin and (±)-catechin, a 50 : 50 mixture of (+)-catechin and (-)-catechin) could protect SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP(+) ) toxicity by decreasing the generation of oxygen free radicals. The inhibitive effect of (±)-catechin on JNK/c-Jun activation was investigated. Methods The effects of (+)-catechin, (-)-catechin or (±)-catechin in protecting against MPP(+) toxicity were evaluated and compared in SH-SY5Y cells by testing the release of lactate dehydrogenase. The generation of reactive oxygen species (ROS) was measured by immunochemistry and the phosphorylation level of JNK/c-Jun was determined by Western blotting. Key findings In SH-SY5Y cells, (+)-catechin, (-)-catechin or (±)-catechin reduced apoptosis induced by MPP(+) and decreased ROS generation caused by MPP(+) . Different enantiomers of catechin showed protective effects at similar potency. Moreover (±)-catechin decreased JNK/c-Jun phosphorylation which was increased by MPP(+) . Conclusions Catechin and its two enantiomers could protect SH-SY5Y cells against MPP(+) cytotoxicity at a similar potency. Antioxidative stress and inhibition of the JNK/c-Jun signalling pathway might have been involved in the neuroprotective mechanisms of catechin against MPP(+) cytotoxicity in SH-SY5Y cells. |
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Authors:
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Hao-Lan Ruan; Yi Yang; Xiao-Nan Zhu; Xue-Lan Wang; Ru-Zhu Chen |
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Publication Detail:
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Type: Journal Article Date: 2011-06-21 |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 63 ISSN: 0022-3573 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 1169-74 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. JPP © 2011 Royal Pharmaceutical Society. |
Affiliation:
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Guangdong Institute for Drug Control, Guangzhou, China Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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