| Similar changes of gene expression in human skeletal muscle after resistance exercise and multiple fine needle biopsies. | |
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MedLine Citation:
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PMID: 22052872 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Repeated biopsy sampling from one muscle is necessary to investigate muscular adaptation to different forms of exercise as adaptation is thought to be the result of cumulative effects of transient changes in gene expression in response to single exercise bouts. In a cross-over study, we obtained 4 fine needle biopsies from one vastus lateralis muscle of 11 male subjects (25.9 ± 3.8 yr, 179.2 ± 4.8 cm, 76.5 ± 7.0 kg), taken before (baseline), 1, 4 and 24 h after one bout of squatting exercise performed as conventional squatting or as whole body vibration exercise . In order to investigate if the repeated biopsy sampling has a confounding effect on the observed changes in gene expression, 4 fine needle biopsies from one vastus lateralis muscle were also taken from 8 male non-exercising control subjects (24.5 ± 3.7 yr, 180.6 ± 1.2 cm, 81.2 ± 1.6 kg) at the equivalent time points. Using RT-PCR, we observed similar patterns of change in the squatting as well as in the control group for the mRNAs of interleukin 6 (IL-6), IL-6 receptor (IL-6R), insulin like growth factor 1 (IGF-1), p21, phosphofructokinase (PFK) and glucose tansporter (GLUT) in relation to the baseline biopsy. In conclusion, multiple fine needle biopsies obtained from the same muscle region can per se influence the expression of marker genes induced by an acute bout of resistance exercise. |
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Authors:
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Birgit Friedmann-Bette; Fides Regina Schwartz; Holger Eckhardt; Rudolf Billeter; Gabriel Bonaterra; Ralf Kinscherf |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-3 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University Hospital. |
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