Document Detail


Similar changes of gene expression in human skeletal muscle after resistance exercise and multiple fine needle biopsies.
MedLine Citation:
PMID:  22052872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Repeated biopsy sampling from one muscle is necessary to investigate muscular adaptation to different forms of exercise as adaptation is thought to be the result of cumulative effects of transient changes in gene expression in response to single exercise bouts. In a crossover study, we obtained four fine needle biopsies from one vastus lateralis muscle of 11 male subjects (25.9 ± 3.8 yr, 179.2 ± 4.8 cm, 76.5 ± 7.0 kg), taken before (baseline), 1, 4, and 24 h after one bout of squatting exercise performed as conventional squatting or as whole body vibration exercise. To investigate if the repeated biopsy sampling has a confounding effect on the observed changes in gene expression, four fine needle biopsies from one vastus lateralis muscle were also taken from 8 male nonexercising control subjects (24.5 ± 3.7 yr, 180.6 ± 1.2 cm, 81.2 ± 1.6 kg) at the equivalent time points. Using RT-PCR, we observed similar patterns of change in the squatting as well as in the control group for the mRNAs of interleukin 6 (IL-6), IL-6 receptor, insulin-like growth factor 1, p21, phosphofructokinase, and glucose transporter in relation to the baseline biopsy. In conclusion, multiple fine needle biopsies obtained from the same muscle region can per se influence the expression of marker genes induced by an acute bout of resistance exercise.
Authors:
Birgit Friedmann-Bette; Fides Regina Schwartz; Holger Eckhardt; Rudolf Billeter; Gabriel Bonaterra; Ralf Kinscherf
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2011-11-03
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  112     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-18     Completed Date:  2013-07-08     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  289-95     Citation Subset:  IM    
Affiliation:
Department of Sports Medicine, Medical Clinic, University Hospital Heidelberg, Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adult
Artifacts*
Biopsy, Fine-Needle / adverse effects*
Exercise / physiology*
Gene Expression*
Humans
Male
Muscle, Skeletal / physiology*
Resistance Training*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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