Document Detail


Similar bacterial community composition in acidic mining lakes with different pH and lake chemistry.
MedLine Citation:
PMID:  20495796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As extreme environmental conditions strongly affect bacterial community composition (BCC), we examined whether differences in pH-even at low pH-and in iron and sulfate concentrations lead to changes in BCC of acidic mining lakes. Thereby, we tested the following hypotheses: (1) diversity of the bacterial community in acidic lakes decreases with reducing pH, (2) BCC differs between epilimnion and hypolimnion, and (3) BCC in extremely acidic environments does not vary much over time. Therefore, we investigated the BCC of three acidic lakes with different pH values (2.3, 2.7, and 3.2) by denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of DGGE bands as well as catalyzed reporter deposition-FISH (CARD-FISH). BCC did not significantly vary among the studied lakes nor differ much between water layers. In contrast, BCC significantly changed over time, which is contradictory to our hypotheses. Bacterial communities were dominated by Alpha-, Beta-, and Gammaproteobacteria, whereas Actino- and Acidobacteria rarely occurred. Cell numbers of both free and attached bacteria were positively related to DOC concentration. Overall, low pH and extreme chemical conditions of the studied lakes led to similar assemblages of bacteria with pronounced temporal differences. This notion indicates that temporal changes in environmental conditions including food web structure also affect unique communities of bacteria thriving at low pH.
Authors:
Heike Kampe; Claudia Dziallas; Hans-Peter Grossart; Norbert Kamjunke
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Publication Detail:
Type:  Journal Article     Date:  2010-05-22
Journal Detail:
Title:  Microbial ecology     Volume:  60     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-14     Completed Date:  2011-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  618-27     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry and Biology, University of Potsdam, Am Neuen Palais 10, 14469 Potsdam, Germany.
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MeSH Terms
Descriptor/Qualifier:
Acids / analysis
Bacteria / genetics*,  isolation & purification
Biota
Carbon / analysis
DNA, Bacterial / genetics
Denaturing Gradient Gel Electrophoresis
Fresh Water / chemistry
Hydrogen-Ion Concentration
Mining*
Oxygen / analysis
Phylogeny
Sequence Analysis, DNA
Temperature
Water Microbiology*
Chemical
Reg. No./Substance:
0/Acids; 0/DNA, Bacterial; 7440-44-0/Carbon; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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