Document Detail

Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
MedLine Citation:
PMID:  225566     Owner:  NLM     Status:  MEDLINE    
In addition to the virus-coded large-T and small-t antigens, two new classes of proteins were immunoprecipitated by anti-simian virus 40 (SV40) tumor serum from extracts of various SV40-transformed cell lines. These were as follows: (i) proteins (termed "super-T proteins") with an Mr higher than that of large-T antigen (86,000), which were found in many SV40-transformed cell lines derived from mouse and rat cells (super-T proteins and large-T antigen appeared to have closely related structures as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides); (ii) proteins (termed "55K proteins") with an Mr ranging from 50,000 to 60,000, which were present in all SV40-transformed cell lines examined so far, including those obtained by chromosome-mediated gene transfer. The 55K proteins were not structurally related to large-T antigens, as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides. Our data are compatible with the assumption that the 55K proteins are largely or totally cell coded.
M Kress; E May; R Cassingena; P May
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  31     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1979 Aug 
Date Detail:
Created Date:  1979-11-21     Completed Date:  1979-11-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  472-83     Citation Subset:  IM    
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MeSH Terms
Antigens, Neoplasm / analysis*
Antigens, Viral / analysis*
Cell Line
Cell Transformation, Neoplastic
Cell Transformation, Viral
Immune Sera
Lung / embryology
Neoplasms, Experimental / immunology
Simian virus 40 / analysis,  immunology*
Viral Proteins / analysis,  immunology*
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Antigens, Viral; 0/Immune Sera; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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