Document Detail


Simian virus 40 small t antigen activates the carboxyl-terminal transforming p53-binding domain of large T antigen.
MedLine Citation:
PMID:  8794316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of the simian virus 40 large T antigen (large T) in F111 rat fibroblasts generated only minimal transformants (e.g., F5 cells). Interestingly, F111-derived cells expressing only an amino-terminal fragment of large T spanning amino acids 1 to 147 (e.g., FR3 cells), revealed the same minimal transformed phenotype as F111 cells expressing full-length large T. This suggested that in F5 cells the transforming domain of large T contained within the C-terminal half of the large T molecule, and spanning the p53 binding domain, was not active. Progression to a more transformed phenotype by coexpression of small t antigen (small t) could be achieved in F5 cells but not in FR3 cells. Small-t-induced progression of F5 cells correlated with metabolic stabilization of p53 in complex with large T: whereas in F5 cells the half-life of p53 in complex with large T was only slightly elevated compared with that of (uncomplexed) p53 in parental F111 cells or that in FR3 cells, coexpression of small t in F5 cells led to metabolic stabilization and to high-level accumulation of p53 complexed to large T. In contrast, coexpression of small t had no effect on p53 stabilization or accumulation in FR3 cells. This finding strongly supports the assumption that the mere physical interaction of large T with p53, and thus p53 inactivation, in F5 cells expressing large T only does not reflect the main transforming activity of the C-terminal transforming domain of large T. In contrast, we assume that the transforming potential of this domain requires activation by a cellular function(s) which is mediated by small t and correlates with metabolic stabilization of p53.
Authors:
J Zerrahn; F Tiemann; W Deppert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  70     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-22     Completed Date:  1996-11-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6781-9     Citation Subset:  IM    
Affiliation:
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Viral, Tumor / metabolism*
Cell Transformation, Viral*
Fibroblasts / virology
Phosphorylation
Rats
Sequence Analysis
Simian virus 40 / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Viral, Tumor
Comments/Corrections

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