Document Detail


Simian virus 40 large T-antigen expression decreases the G1 and increases the G2 + M cell cycle phase durations in exponentially growing cells.
MedLine Citation:
PMID:  1309888     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of simian virus 40 large T-antigen (Tag) expression on the cell cycle of exponentially growing, established, mouse NIH 3T3 fibroblasts was examined by using a sensitive flow cytometric assay to analyze nonselected cells immediately after infection with a Tag-encoding recombinant retrovirus. Tag expression resulted in reduced percentages of G1-phase cells and increased percentages of S- and G2 + M-phase cells compared with cell populations infected with a control virus not encoding the Tag gene. Cell cycle-blocking drugs were used to examine the exit rate for each of the cell cycle phases, G1, S, and G2 + M, for Tag-expressing and Tag-nonexpressing cells growing in the same cell culture dish. As a result of Tag expression, the duration of the G1 phase was decreased (average G1-phase exit duration decreased by 18%) and the duration of the G2 + M phase was increased (average G2 + M exit duration increased by 29%). The duration of S phase was unaffected by Tag expression.
Authors:
T L Sladek; J W Jacobberger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-14     Completed Date:  1992-02-14     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1059-65     Citation Subset:  IM    
Affiliation:
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Antigens, Polyomavirus Transforming / genetics*
Cell Cycle* / drug effects
Cell Division
G1 Phase
G2 Phase
Helper Viruses / genetics,  physiology
Kinetics
Mice
Mitosis
Nocodazole / pharmacology
Simian virus 40 / genetics,  physiology*
Virus Replication
Grant Support
ID/Acronym/Agency:
CA43703/CA/NCI NIH HHS; HL41945/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming; 31430-18-9/Nocodazole
Comments/Corrections

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