Document Detail

Simian virus 40 induces multiple S phases with the majority of viral DNA replication in the G2 and second S phase in CV-1 cells.
MedLine Citation:
PMID:  10912803     Owner:  NLM     Status:  MEDLINE    
The infection of permissive monkey kidney cells (CV-1) with simian virus 40 induces G1 growth-arrested cells into the cell cycle. After completion of the first S phase and movement into G2, mitosis was blocked and the cells entered another DNA synthesis cycle (second S phase). Growth-arrested CV-1 cells replicated significant amounts of viral DNA in the G2 phase with the majority of synthesis occurring during the second S phase. When mimosine-blocked (G1/S) infected cells were released into the cell cycle, a major portion of the viral DNA was detected in G2 with the largest accumulation in the second S phase. The total DNA produced per infected cell was 10-12C with approximately 0.5-2C of viral DNA replicated per cell. Therefore the majority of the DNA per cell was cellular, 4C from the first S phase and approximately 4-6C from the second cellular synthesis phase.
J M Lehman; J Laffin; T D Friedrich
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  258     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-08     Completed Date:  2000-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  215-22     Citation Subset:  IM    
Department of Microbiology, Immunology, and Molecular Genetics, Center for Immunology and Microbial Diseases, Albany Medical College, New York 12208, USA.
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MeSH Terms
Cell Cycle* / drug effects
Cell Division
Cell Line
Centrosome / ultrastructure
Cercopithecus aethiops
DNA Replication*
DNA, Viral / analysis
G2 Phase
Mimosine / pharmacology
S Phase
Simian virus 40 / genetics*,  physiology
Virus Replication
Grant Support
Reg. No./Substance:
0/DNA, Viral; 500-44-7/Mimosine

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