| Simian virus 40 gene A regulation of cellular DNA synthesis. I. In permissive cells. | |
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MedLine Citation:
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PMID: 224217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The kinetics of host cellular DNA stimulation by simian virus 40 (SV40) tsA58 infection was studied by flow microfluorometry and autoradiography in two types of productively infected monkey kidney cells (AGMK, secondary passage, and the TC-7 cell line). Prior to infection, the cell populations were maintained predominantly in G0-G1 hase of the cell cycle by low (0.25%) serum concentration. Infection of TC-7 or AGMK cells by wild-type SV40, viable deletion mutant dl890, or by SV40 tsA58 at 33 degrees C induced cells through S phase after which they were blocked with a 4N DNA content in the G2 phase. The infection of TC-7 cells by tsA58 at 41 degrees C, which was a nonpermissive temperature for viral DNA replication, induced a round of cell DNA synthesis in approximately 30% of the cell population. These cells proceeded through S phase but then re-entered the G1 resting state. In contrast, infection of AGMK cells by tsA58 at 41 degrees C induced DNA synthesis in approximately 50% of the cells, but this population remained blocked in the G2 phase. These results indicate that the mitogenic effect of the A gene product upon cellular DNA is more heat resistant than its regulating activity on viral DNA synthesis and that the extent of induction of cell DNA synthesis by the A gene product may be influenced by the host cell. |
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Authors:
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J B Hiscott; V Defendi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 30 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1979 May |
Date Detail:
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Created Date: 1979-10-24 Completed Date: 1979-10-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 590-9 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Cell Line DNA / biosynthesis* DNA, Viral / biosynthesis Genes, Viral* Kinetics Simian virus 40 / genetics*, metabolism Temperature Viral Proteins / physiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Viral Proteins; 9007-49-2/DNA |
| Comments/Corrections | |
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