| Simian virus 40 functions required for the establishment and maintenance of malignant transformation. | |
| | |
MedLine Citation:
|
PMID: 163374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Members of the five classes of temperature-sensitive simian virus 40 mutants were tested for their ability to transform Chinese hamster lung cells. Two criteria for transformation were used: the ability to form clones in medium with low serum concentrations and the ability to overgrow a monolayer. Only A mutants failed to transform at the restrictive temperature when subconfluent Chinese hamster lung monolayers were used. However, both A and D mutants failed to transform at the restrictive temperature when confluent monolayers and depleted medium were used. When transformed clones were selected, purified by recloning, and examined at both temperatures, only cell lines induced by A mutants lost the transformed phenotype at the higher temperature. Thus, A function is required for maintenance of the transformed phenotype in Chinese hamster lung cells. A function is known to be required for the initiation of viral DNA synthesis in permissive cells. Therefore, transformation may be a consequence of the introduction into a cell of the capacity for aberrant initiation of DNA replication. |
| | |
Authors:
|
R G Martin; J Y Chou |
Related Documents
:
|
4352654 - Membrane changes and adenosine triphosphate content in normal and malignant transformed... 8396814 - The products of the e5, e6, or e7 open reading frames of rhpv 1 can individually transf... 6825074 - Analysis of the interaction between rauscher murine leukemia virus and murine cell memb... 16316754 - Budded karyoplasts from multinucleated fibroblast cells contain centrosomes and change ... 20399854 - Long-term genistein treatment of mcf-7 cells decreases acetylated histone 3 expression ... 10854604 - Fractionation of differentiating cells using density perturbation. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of virology Volume: 15 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1975 Mar |
Date Detail:
|
Created Date: 1975-05-21 Completed Date: 1975-05-21 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 599-612 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adsorption Animals Cell Line Cell Membrane / microbiology Cell Transformation, Neoplastic* Clone Cells Cricetinae Culture Media Genes* Genetic Complementation Test Lung Mutation Phenotype Simian virus 40 / growth & development* Temperature |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA of Epstein-Barr virus. I. Comparative studies of the DNA of Epstein-Barr virus from HR-1 and B95...
Next Document: Function of simian virus 40 gene A in transforming infection.