Document Detail

Simian virus 40 functions required for the establishment and maintenance of malignant transformation.
MedLine Citation:
PMID:  163374     Owner:  NLM     Status:  MEDLINE    
Members of the five classes of temperature-sensitive simian virus 40 mutants were tested for their ability to transform Chinese hamster lung cells. Two criteria for transformation were used: the ability to form clones in medium with low serum concentrations and the ability to overgrow a monolayer. Only A mutants failed to transform at the restrictive temperature when subconfluent Chinese hamster lung monolayers were used. However, both A and D mutants failed to transform at the restrictive temperature when confluent monolayers and depleted medium were used. When transformed clones were selected, purified by recloning, and examined at both temperatures, only cell lines induced by A mutants lost the transformed phenotype at the higher temperature. Thus, A function is required for maintenance of the transformed phenotype in Chinese hamster lung cells. A function is known to be required for the initiation of viral DNA synthesis in permissive cells. Therefore, transformation may be a consequence of the introduction into a cell of the capacity for aberrant initiation of DNA replication.
R G Martin; J Y Chou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  15     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1975 Mar 
Date Detail:
Created Date:  1975-05-21     Completed Date:  1975-05-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  599-612     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Membrane / microbiology
Cell Transformation, Neoplastic*
Clone Cells
Culture Media
Genetic Complementation Test
Simian virus 40 / growth & development*
Reg. No./Substance:
0/Culture Media

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