Document Detail


Simian T-lymphotropic Virus-associated lymphoma in 2 naturally infected baboons: T-cell clonal expansion and immune response during tumor development.
MedLine Citation:
PMID:  23759532     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two young female baboons naturally infected with simian T-lymphotropic virus type 1 (STLV1) were euthanized due to chronic respiratory disease that was unresponsive to treatment. Massive lymphocytic infiltration of the lung interstitium suggested a diagnosis of STLV-associated lymphoma. In each case, the diagnosis was confirmed through inverse PCR (IPCR) that detected monoclonally integrated STLV1 provirus in cellular DNA extracted from lymphoma tissue and peripheral blood cells (PBC). One dominant STLV1-infected T-cell clone and 3 minor clones were detected in PBC from each baboon. Using archived PBC DNA and primers within the proviral genome and chromosomal DNA flanking the STLV1 integration sites in PCR analyses, we determined that the dominant clone in one baboon had first appeared approximately 8 mo after infection and had circulated for 4 y before clinical disease developed. ELISA testing of archived serum revealed that both baboons seroconverted to the p19 and p24 gag proteins and the envelope gp46 protein but not to the viral tax protein. Titers to p24 and gp46 rose significantly after infection and remained relatively constant until death, whereas titers to p19 increased with time. Although spontaneous STLV1-associated lymphomas have been described in baboons, the STLV1-associated lymphomas described here occurred in 2 relatively young baboons, both of whom had become infected with STLV at 3 to 4 y of age and developed lymphoma within 5 y of infection.
Authors:
Jean M d'Offay; Richard Eberle; Roman F Wolf; Stanley D Kosanke; Kelly R Doocy; Sahlu Ayalew; Keith G Mansfeild; Gary L White
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Comparative medicine     Volume:  63     ISSN:  1532-0820     ISO Abbreviation:  Comp. Med.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-13     Completed Date:  2014-01-15     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  100900466     Medline TA:  Comp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  288-94     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Primers
Enzyme-Linked Immunosorbent Assay
Female
Genes, Viral
Lymphoma / immunology,  virology*
Papio
Polymerase Chain Reaction / methods
Simian T-lymphotropic virus 1 / genetics,  isolation & purification,  pathogenicity*
Viral Load
Grant Support
ID/Acronym/Agency:
P40 OD010431/OD/NIH HHS; P40 OD010988/OD/NIH HHS; P40 RR012317/RR/NCRR NIH HHS; P40 RR12317/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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