| Simian immunodeficiency virus infection alters chemokine networks in lung tissues of cynomolgus macaques: association with Pneumocystis carinii infection. | |
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MedLine Citation:
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PMID: 20671263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infection by HIV-1 frequently leads to pulmonary complications, including alterations to local immune environments. To better understand these alterations, we have examined in detail the patterns and levels of expression of chemokine, cytokine, and chemokine receptor mRNAs in lung tissues from 16 uninfected or simian immunodeficiency virus (SIV)/DeltaB670 infected cynomolgus macaques at different stages of infection. Among the most up-regulated immune genes were interferon (IFN)-gamma, IFN-gamma-inducible CXCR3 ligands, and CCR5 ligands, as well as the cognate chemokine receptors. These changes were greatest in animals with clear Pneumocystis carinii coinfection. Immunohistochemistry and in situ hybridization revealed monocytes/macrophages to be the predominant type of cell infiltrating into lung tissues and serving as the major cellular source of chemokines. To explore the causes of chemokine alterations, we treated macaque lung cells with IFN-gamma, lipopolysaccharide, Poly(I:C), and P. carinii in vitro, and results revealed that these stimuli can induce the expression of CXCR3 ligand and/or CCR5 ligand mRNAs. Taken together, these studies provide a comprehensive definition of the chemokine networks available to modulate cellular recruitment to lung tissues during SIV infection and implicate both cytokines (IFN-gamma) and pathogens (SIV and P. carinii) as contributors to increased expression of pro-inflammatory chemokines. |
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Authors:
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Shulin Qin; Beth A Fallert Junecko; Anita M Trichel; Patrick M Tarwater; Michael A Murphey-Corb; Denise E Kirschner; Todd A Reinhart |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-29 |
Journal Detail:
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Title: The American journal of pathology Volume: 177 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-31 Completed Date: 2010-12-13 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 1274-85 Citation Subset: AIM; IM |
Affiliation:
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Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15261, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemokines / immunology*, metabolism Immunohistochemistry In Situ Hybridization Lung / immunology*, metabolism, virology Macaca fascicularis Pneumocystis carinii / immunology* Pneumonia, Pneumocystis / complications, immunology*, metabolism, virology Receptors, Chemokine / immunology*, metabolism Reverse Transcriptase Polymerase Chain Reaction Simian Acquired Immunodeficiency Syndrome / complications, immunology*, metabolism, virology Simian immunodeficiency virus / immunology* Statistics, Nonparametric |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI060422/AI/NIAID NIH HHS; R01 HL072682/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Receptors, Chemokine |
| Comments/Corrections | |
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