Document Detail


Sim1a and Arnt2 contribute to hypothalamo-spinal axon guidance by regulating Robo2 activity via a Robo3-dependent mechanism.
MedLine Citation:
PMID:  23222439     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Precise spatiotemporal control of axon guidance factor expression is a prerequisite for formation of functional neuronal connections. Although Netrin/Dcc- and Robo/Slit-mediated attractive and repulsive guidance of commissural axons have been extensively studied, little is known about mechanisms controlling mediolateral positioning of longitudinal axons in vertebrates. Here, we use a genetic approach in zebrafish embryos to study pathfinding mechanisms of dopaminergic and neuroendocrine longitudinal axons projecting from the hypothalamus into hindbrain and spinal cord. The transcription factors Sim1a and Arnt2 contribute to differentiation of a defined population of dopaminergic and neuroendocrine neurons. We show that both factors also control aspects of axon guidance: Sim1a or Arnt2 depletion results in displacement of hypothalamo-spinal longitudinal axons towards the midline. This phenotype is suppressed in robo3 guidance receptor mutant embryos. In the absence of Sim1a and Arnt2, expression of the robo3 splice isoform robo3a.1 is increased in the hypothalamus, indicating negative control of robo3a.1 transcription by these factors. We further provide evidence that increased Robo3a.1 levels interfere with Robo2-mediated repulsive axon guidance. Finally, we show that the N-terminal domain unique to Robo3a.1 mediates the block of Robo2 repulsive activity. Therefore, Sim1a and Arnt2 contribute to control of lateral positioning of longitudinal hypothalamic-spinal axons by negative regulation of robo3a.1 expression, which in turn attenuates the repulsive activity of Robo2.
Authors:
Jörn Schweitzer; Heiko Löhr; Joshua L Bonkowsky; Katrin Hübscher; Wolfgang Driever
Related Documents :
23396189 - Gli3 is required in emx1+ progenitors for the development of the corpus callosum.
23592129 - Melanocortin-4 receptor expression in the rostral ventromedial medulla involved in modu...
23817549 - Inhibition of inhibition in visual cortex: the logic of connections between molecularly...
24405769 - Cystatin c triggers neuronal degeneration in a model of multiple system atrophy.
22940549 - Gaba(a) receptor-mediated feedforward and feedback inhibition differentially modulates ...
24227729 - Uncoupling of epha/ephrina signaling and spontaneous activity in neural circuit wiring.
11323519 - Evidence for the involvement of par-4 in ischemic neuron cell death.
7976549 - Mechanisms of brain injury associated with partial and complete occlusion of the mca in...
21374489 - Use of lectins as transganglionic neuronal tracers in the study of unmyelinated primary...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  140     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  93-106     Citation Subset:  IM    
Affiliation:
Developmental Biology, Institute Biology 1, Faculty of Biology, University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dose-dependent roles for canonical Wnt signalling in de novo crypt formation and cell cycle properti...
Next Document:  JAK/STAT autocontrol of ligand-producing cell number through apoptosis.