| Silver staining of proteins in polyacrylamide gels. | |
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MedLine Citation:
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PMID: 17487168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks. |
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Authors:
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Mireille Chevallet; Sylvie Luche; Thierry Rabilloud |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 1 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2006 |
Date Detail:
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Created Date: 2007-05-09 Completed Date: 2007-10-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 1852-8 Citation Subset: IM |
Affiliation:
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CEA-Grenoble, DRDC/ICH, 38054 Grenoble, Cedex 9, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Electrophoresis, Polyacrylamide Gel Mass Spectrometry Silver Staining / methods* Temperature |
| Comments/Corrections | |
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