Document Detail


Silver staining of proteins in polyacrylamide gels.
MedLine Citation:
PMID:  17487168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
Authors:
Mireille Chevallet; Sylvie Luche; Thierry Rabilloud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  1     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2006  
Date Detail:
Created Date:  2007-05-09     Completed Date:  2007-10-30     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1852-8     Citation Subset:  IM    
Affiliation:
CEA-Grenoble, DRDC/ICH, 38054 Grenoble, Cedex 9, France.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Polyacrylamide Gel
Mass Spectrometry
Silver Staining / methods*
Temperature
Comments/Corrections

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