Document Detail

Silver staining of proteins in polyacrylamide gels.
MedLine Citation:
PMID:  17487168     Owner:  NLM     Status:  MEDLINE    
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation and finally image development. Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks.
Mireille Chevallet; Sylvie Luche; Thierry Rabilloud
Related Documents :
21166718 - Analysis of differentially expressed proteins in oral squamous cell carcinoma by maldi-...
22285518 - Protein structure determination from pseudocontact shifts using rosetta.
2461118 - India ink staining of proteins on nylon and hydrophobic membranes.
3287108 - Simplified method for preparation of concentrated exoproteins produced by staphylococcu...
23702218 - Prenatal alcohol exposure alters the cerebral cortex proteome in weanling rats.
20658338 - Effective 3d protein structure prediction with local adjustment genetic-annealing algor...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  1     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2006  
Date Detail:
Created Date:  2007-05-09     Completed Date:  2007-10-30     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1852-8     Citation Subset:  IM    
CEA-Grenoble, DRDC/ICH, 38054 Grenoble, Cedex 9, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Electrophoresis, Polyacrylamide Gel
Mass Spectrometry
Silver Staining / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative es...
Next Document:  Simultaneous imaging of morphological plasticity and calcium dynamics in dendrites.