Document Detail


Silver staining for nucleolar organizing regions of vertebrate chromosomes.
MedLine Citation:
PMID:  6192554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Refinements to a simple, one-step silver staining technique for nucleolar organizing regions are described. These include fixation of silver stained material with sodium thiosulfate and standardization of silver development conditions for different groups of vertebrates. The central advantages to the method are that it is rapid, reliable, simple, and inexpensive. Additional benefits include (i) consistent and uniform silver staining of nucleolar organizing regions, (ii) few reduced silver deposits elsewhere on the chromosomes or on the slides, (iii) generally unaltered chromosome morphology after silver treatment, and (iv) relative permanence of Permounted preparations. The method works equally well on chromosomes made from cell cultures and from solid tissues of live specimens.
Authors:
J R Gold; J R Ellison
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Stain technology     Volume:  58     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-09-20     Completed Date:  1983-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chiroptera
Chromosomes / ultrastructure*
Fishes
Humans
Metaphase
Nucleolus Organizer Region / ultrastructure*
Silver / diagnostic use*
Staining and Labeling
Turtles
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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