Document Detail

Silver staining in clinical cytogenetics.
MedLine Citation:
PMID:  2412316     Owner:  NLM     Status:  MEDLINE    
Silver staining of human chromosomes at prometaphase or metaphase identifies variants in the stalk (nucleolar organizing) regions of acrocentric chromosomes (Nos. 13, 14, 15, 21, 22). Variants are defined by size, number, and morphology of silver staining areas. They are heritable polymorphisms and have not been associated with clinical abnormalities. However, these variants are useful in clinical cytogenetics, specifically in studies attempting to determine whether genetic material has been gained or lost in chromosomal rearrangements, the origin of chromosomal aberrations, the origin of cells in tissue culture, the chromosomal location of single genes, clonal origin of tumors, the zygosity of twins, and paternity. Some chromosomal aberrations require silver staining for their definition. Because loss of the stalk regions per se is apparently not deleterious, demonstration that chromosomal breaks occurred within this region without concomitant loss or gain of genetic material essential for normal human development provides basis for a good prognosis for the individual with the chromosomal rearrangement resulting from such breakage. The principle underlying most of the other applications is to determine whether variants being compared are identical or dissimilar, and to make inferences from these results (e.g., variants in monozygotic twins should all be identical, whereas in dizygotic twins they are as similar as in any pair of sibs). Silver staining is a valuable technique for special questions in clinical analysis.
A O Martin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  60     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-10-08     Completed Date:  1985-10-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  275-84     Citation Subset:  IM    
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MeSH Terms
Chromosome Aberrations* / genetics
Chromosome Banding
Chromosome Disorders*
Genetic Markers
Silver / diagnostic use*
Staining and Labeling
Reg. No./Substance:
0/Genetic Markers; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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