Document Detail


Silver staining of histone-depleted metaphase chromosomes.
MedLine Citation:
PMID:  6193978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate a possible relationship between the core-like structures seen in silver-stained chromosomes (prepared by standard cytogenetic methods) and the scaffolds observed in histone-depleted chromosomes, the ability of the scaffold to stain with silver has been examined. Isolated chromosomes were histone-depleted by washing in ammonium acetate or by spreading the chromosomes on an ammonium acetate hypophase. The residual chromosome structures were carbon-platinum shadowed or stained with silver, and then examined by electron microscopy. The results provide clear evidence that the scaffold structure has a high affinity for silver and is therefore similar in its silver-staining potential to the core structure in standard chromosomes. This suggests that the silver core in standard chromosomes may represent the scaffold visualized by histone depletion. The peripherally dispersed DNA radiating from the scaffold also proved to be silver-reactive, and additional experiments demonstrated that purified DNA is capable of binding silver. This result indicates that cytological silver staining is not simply a matter of staining protein, as has previously been thought, but may also involve the staining of chromosomal DNA. In the ammonium acetate-treated and carbon-platinum-shadowed preparations, the scaffold structure was highly variable in its morphology and appeared to be composed of undispersed or incompletely dehistonized chromatin fibers. The silver-stained scaffold reflected this variability. Taken together with other evidence, these findings lead to a questioning of the reality of chromosome core structures.
Authors:
G D Burkholder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  147     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  287-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetates
Animals
Cell Line
Chromosomes / analysis,  ultrastructure*
Cricetinae
DNA / analysis
Female
Histones / analysis*
Metaphase*
Microscopy, Electron
Ovary
Silver Nitrate
Staining and Labeling
Chemical
Reg. No./Substance:
0/Acetates; 0/Histones; 631-61-8/ammonium acetate; 7761-88-8/Silver Nitrate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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