Document Detail

Silver staining of collagen type I after sodium dodecylsulphate polyacrylamide gel electrophoresis: effect of Maillard reaction.
MedLine Citation:
PMID:  1400786     Owner:  NLM     Status:  MEDLINE    
Differences in the acidic silver staining, after sodium dodecylsulphate polyacrylamide gel electrophoresis, between the alpha 1 and alpha 2 collagen chains, as well as between rat-tail tendon and calf-skin collagen type I, were observed. The staining conditions at which the staining differences are both most expressed and reproducible were characterized. Age differences between staining of the alpha 1 CB6 fragment from young rats (2 and 12 months) and old rats (29 months) indicated that different susceptibilities of collagen species to the silver staining can be the result of different extents of some age-dependent post-translational modification, such as glycation. In vitro incubation of acid-soluble rat-tail tendon collagen with various sugars led to an increase in silver staining compared with samples incubated in the absence of sugar. This effect was inhibited by sodium cyanoborohydride, diethylenetriamine pentaacetic acid and aminoguanidine, i.e. compounds inhibiting the Maillard reaction at various stages. It can be concluded that the enhanced silver susceptibility of glycated collagen is related to advanced-phase Maillard reaction products attached to collagen.
Z Hodny; R Struzinsky; Z Deyl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  578     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-11-23     Completed Date:  1992-11-23     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
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MeSH Terms
Collagen / analysis*
Electrophoresis, Polyacrylamide Gel / methods
Maillard Reaction*
Silver Staining*
Reg. No./Substance:
0/Carbohydrates; 9007-34-5/Collagen

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