Document Detail


Silver-stained fibrin zymography: separation of proteases and activity detection using a single substrate-containing gel.
MedLine Citation:
PMID:  21487781     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved.
Authors:
Dong-Min Chung; Ki Eun Kim; Keug-Hyun Ahn; Chan-Sun Park; Dong-Ho Kim; Hong Bum Koh; Hyo Kon Chun; Byung-Dae Yoon; Hong Jib Kim; Min-Soo Kim; Nack-Shick Choi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-13
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute Bioindustry Research Center, KRIBB, Jeonbuk, 580-185, Korea.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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