Document Detail


Silver stained core-like structures in chinese hamster metaphase Chromosomes.
MedLine Citation:
PMID:  6165446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chinese hamster metaphase chromosomes, subjected to prolonged hypotonic pretreatment and subsequently stained with ammoniacal silver, contained a darkly-stained core-like structure in each chromatid, surrounded by a halo of dispersed chromatin which was pale yellow to brown in color. The core was variable in its appearance, ranging from a continuous linear configuration to a spiral structure or a discontinuous, particulate structure. Within the centromeric regions, the cores frequently appeared more intensely stained than elsewhere in the chromosome. The nucleolus organizers also stained darkly and appeared to be attached to the core-like structures. It remains to be determined whether the cores represent a real component of metaphase chromosome structure, or whether they are artifacts resulting from abnormal chromatin aggregation arising at the time of chromosome preparation.
Authors:
M Z Kaiserman; G D Burkholder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie     Volume:  22     ISSN:  0008-4093     ISO Abbreviation:  Can. J. Genet. Cytol.     Publication Date:  1980  
Date Detail:
Created Date:  1981-08-20     Completed Date:  1981-08-20     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0372706     Medline TA:  Can J Genet Cytol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  627-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Centromere / ultrastructure
Chromatids / ultrastructure
Chromatin / ultrastructure
Chromosomes / ultrastructure*
Cricetinae
Metaphase
Nucleolus Organizer Region / ultrastructure
Silver
Staining and Labeling
Chemical
Reg. No./Substance:
0/Chromatin; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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