Document Detail

Silver nanoparticles-mediated G2/M cycle arrest of renal epithelial cells is associated with NRF2-GSH signaling.
MedLine Citation:
PMID:  22546375     Owner:  NLM     Status:  Publisher    
Silver nanoparticles (nAg) are known to evoke reactive oxygen species (ROS) generation and consequent cell damage. The transcription factor NF-E2-related factor 2 (NRF2) controls both the basal and inducible expression of multiple antioxidant genes. This study was aimed to investigate the role of NRF2 in nAg-induced renal epithelial cell damage. nAg treatment intensified DNA damage and G2/M cell cycle arrest by nAg in NRF2 knockdown HK-2 (NRF2i) compared with the control cells. As a signaling mechanism associated with nAg-mediated growth arrest, the levels of phospho-CDC25C and phospho-CDC2 were significantly increased in NRF2i. Target gene analysis revealed that nAg-mediated increase in γ-glutamate cysteine ligase expression is NRF2-dependent: nAg-treated NRF2i showed a reduction in glutathione (GSH) content and elevation in ROS level in comparison with the control cells. Additionally, pretreatment of N-acetylcystein in nAg-treated NRF2i alleviated ROS-mediated DNA damage and G2/M cell cycle arrest, while GSH depletion exacerbated DNA damage and cell cycle arrest in the control cells. Taken together, these results suggest that NRF2-mediated GSH increase plays a protective role in nAg-induced DNA damage and subsequent G2/M cell cycle arrest in human renal epithelial cells.
Su Jin Kang; Young Joon Lee; Eun-Kyung Lee; Mi-Kyoung Kwak
Related Documents :
20656205 - Cell migration: mim takes the driver's seat.
15465325 - Carbohydrate-carbohydrate interactions in cell recognition.
22438615 - Urothelial cells in smears from cervix uteri.
19551525 - The molecular mechanism of induced pluripotency: a two-stage switch.
10149425 - Evaluation of the cell-dyn 1600 in an outpatient laboratory.
20523825 - Reduced mitochondrial properties in putative progenitor/stem cells of human keratinocytes.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-27
Journal Detail:
Title:  Toxicology letters     Volume:  -     ISSN:  1879-3169     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Daegu Haany University, College of Oriental Medicine, Gyeongsan-si, Gyeongsangbuk-do 712-715, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The choice of the intravenous fluid influences the tolerance of acute normovolemic anemia in anesthe...
Next Document:  Altering gait by way of stimulation of the plantar surface of the foot: the immediate effect of wear...