| Silver nanoparticles-mediated G2/M cycle arrest of renal epithelial cells is associated with NRF2-GSH signaling. | |
| | |
MedLine Citation:
|
PMID: 22546375 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Silver nanoparticles (nAg) are known to evoke reactive oxygen species (ROS) generation and consequent cell damage. The transcription factor NF-E2-related factor 2 (NRF2) controls both the basal and inducible expression of multiple antioxidant genes. This study was aimed to investigate the role of NRF2 in nAg-induced renal epithelial cell damage. nAg treatment intensified DNA damage and G2/M cell cycle arrest by nAg in NRF2 knockdown HK-2 (NRF2i) compared with the control cells. As a signaling mechanism associated with nAg-mediated growth arrest, the levels of phospho-CDC25C and phospho-CDC2 were significantly increased in NRF2i. Target gene analysis revealed that nAg-mediated increase in γ-glutamate cysteine ligase expression is NRF2-dependent: nAg-treated NRF2i showed a reduction in glutathione (GSH) content and elevation in ROS level in comparison with the control cells. Additionally, pretreatment of N-acetylcystein in nAg-treated NRF2i alleviated ROS-mediated DNA damage and G2/M cell cycle arrest, while GSH depletion exacerbated DNA damage and cell cycle arrest in the control cells. Taken together, these results suggest that NRF2-mediated GSH increase plays a protective role in nAg-induced DNA damage and subsequent G2/M cell cycle arrest in human renal epithelial cells. |
| | |
Authors:
|
Su Jin Kang; Young Joon Lee; Eun-Kyung Lee; Mi-Kyoung Kwak |
Related Documents
:
|
20656205 - Cell migration: mim takes the driver's seat. 15465325 - Carbohydrate-carbohydrate interactions in cell recognition. 22438615 - Urothelial cells in smears from cervix uteri. 19551525 - The molecular mechanism of induced pluripotency: a two-stage switch. 10149425 - Evaluation of the cell-dyn 1600 in an outpatient laboratory. 20523825 - Reduced mitochondrial properties in putative progenitor/stem cells of human keratinocytes. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-27 |
Journal Detail:
|
Title: Toxicology letters Volume: - ISSN: 1879-3169 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-5-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Ireland Ltd. |
Affiliation:
|
Daegu Haany University, College of Oriental Medicine, Gyeongsan-si, Gyeongsangbuk-do 712-715, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The choice of the intravenous fluid influences the tolerance of acute normovolemic anemia in anesthe...
Next Document: Altering gait by way of stimulation of the plantar surface of the foot: the immediate effect of wear...