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Silver nanoparticles exert a long-lasting antiproliferative effect on human keratinocyte HaCaT cell line.
MedLine Citation:
PMID:  21501681     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
For their antibacterial activity, silver nanoparticles (Ag NPs) are largely used in various commercially available products designed to come in direct contact with the skin. In this study we investigated the effects of Ag NPs on skin using the human-derived keratinocyte HaCaT cell line model. Ag NPs caused a concentration- and time-dependent decrease of cell viability, with IC(50) values of 6.8±1.3μM (MTT assay) and 12±1.2μM (SRB assay) after 7days of contact. A 24h treatment, followed by a 6day recovery period in Ag NPs-free medium, reduced cell viability with almost the same potency (IC(50)s of 15.3±4.6 and 35±20μM, MTT and SRB assays, respectively). Under these conditions, no evidence of induction of necrotic events (propidium iodide assay) was found. Apocynin, NADPH-oxidase inhibitor, or N(G)-monomethyl-l-argynine, nitric oxide synthase inhibitor, did not prevent NPs-induced reduction of cell viability. TEM analysis of cells exposed to NPs for 24h revealed alteration of nuclear morphology but only a marginal presence of individual NPs inside the cells. These results demonstrate that on HaCaT keratinocytes a relatively short time of contact with Ag NPs causes a long-lasting inhibition of cell growth, not associated with consistent Ag NPs internalization.
Authors:
Caterina Zanette; Marco Pelin; Matteo Crosera; Gianpiero Adami; Massimo Bovenzi; Francesca Filon Larese; Chiara Florio
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-8
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  -     ISSN:  1879-3177     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Affiliation:
Department of Life Sciences, University of Trieste, Via L. Giorgeri 7/9, 34127 Trieste, Italy.
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