Document Detail


Silicon-based biosensors for rapid detection of protein or nucleic acid targets.
MedLine Citation:
PMID:  11568116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format. METHODS: Capture probes were immobilized either passively or covalently on the optically coated surface of the biosensor. Intermolecular interactions of the immobilized capture probe with specific target molecules were transduced into a molecular thin film. Thin films were generated by enzyme-catalyzed deposition in the vicinity of the surface-bound target. The increased thickness on the surface changed the apparent color of the biosensor by altering the interference pattern of reflected light. RESULTS: Cytokine detection was achieved in a 40-min multiplex assay. Detection limits were 4 ng/L for interleukin (IL)-6, 31 ng/L for IL1-beta, and 437 ng/L for interferon-gamma. In multianalyte experiments, cytokines were specifically detected with signal-to-noise ratios ranging from 15 to 80. With a modified optical surface, specificity was also demonstrated in a nucleic acid array with unambiguous discrimination of single-base changes in a 15-min assay. For homozygous wild-type and homozygous mutant samples, signal-to-noise ratios of approximately 100 were observed. Heterozygous samples yielded approximately equivalent signals for wild-type and mutant capture probes. CONCLUSIONS: The thin-film biosensor allows rapid, sensitive, and specific detection of protein or nucleic acid targets in an array format with results read visually or quantified with a charge-coupled device camera. This biosensor is suited for multianalyte detection in clinical diagnostic assays.
Authors:
R Jenison; H La; A Haeberli; R Ostroff; B Polisky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  47     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-24     Completed Date:  2001-10-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1894-900     Citation Subset:  IM    
Affiliation:
ThermoBioStar, Inc., 6655 Lookout Rd., Boulder, CO 80301, USA.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques*
Cystic Fibrosis Transmembrane Conductance Regulator / genetics
DNA / analysis
Humans
Interferon-gamma / analysis
Interleukin-1 / analysis
Interleukin-6 / analysis
Nucleic Acids / analysis*
Polymorphism, Single Nucleotide
Proteins / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Silicon Compounds*
Chemical
Reg. No./Substance:
0/CFTR protein, human; 0/Interleukin-1; 0/Interleukin-6; 0/Nucleic Acids; 0/Proteins; 0/Silicon Compounds; 12033-89-5/silicon nitride; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 82115-62-6/Interferon-gamma; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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