| Silicon-based biosensors for rapid detection of protein or nucleic acid targets. | |
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MedLine Citation:
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PMID: 11568116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We developed a silicon-based biosensor that generates visual, qualitative results or quantitative results for the detection of protein or nucleic acid targets in a multiplex format. METHODS: Capture probes were immobilized either passively or covalently on the optically coated surface of the biosensor. Intermolecular interactions of the immobilized capture probe with specific target molecules were transduced into a molecular thin film. Thin films were generated by enzyme-catalyzed deposition in the vicinity of the surface-bound target. The increased thickness on the surface changed the apparent color of the biosensor by altering the interference pattern of reflected light. RESULTS: Cytokine detection was achieved in a 40-min multiplex assay. Detection limits were 4 ng/L for interleukin (IL)-6, 31 ng/L for IL1-beta, and 437 ng/L for interferon-gamma. In multianalyte experiments, cytokines were specifically detected with signal-to-noise ratios ranging from 15 to 80. With a modified optical surface, specificity was also demonstrated in a nucleic acid array with unambiguous discrimination of single-base changes in a 15-min assay. For homozygous wild-type and homozygous mutant samples, signal-to-noise ratios of approximately 100 were observed. Heterozygous samples yielded approximately equivalent signals for wild-type and mutant capture probes. CONCLUSIONS: The thin-film biosensor allows rapid, sensitive, and specific detection of protein or nucleic acid targets in an array format with results read visually or quantified with a charge-coupled device camera. This biosensor is suited for multianalyte detection in clinical diagnostic assays. |
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Authors:
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R Jenison; H La; A Haeberli; R Ostroff; B Polisky |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 47 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-09-24 Completed Date: 2001-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1894-900 Citation Subset: IM |
Affiliation:
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ThermoBioStar, Inc., 6655 Lookout Rd., Boulder, CO 80301, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques* Cystic Fibrosis Transmembrane Conductance Regulator / genetics DNA / analysis Humans Interferon-gamma / analysis Interleukin-1 / analysis Interleukin-6 / analysis Nucleic Acids / analysis* Polymorphism, Single Nucleotide Proteins / analysis* Reverse Transcriptase Polymerase Chain Reaction Silicon Compounds* |
| Chemical | |
Reg. No./Substance:
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0/CFTR protein, human; 0/Interleukin-1; 0/Interleukin-6; 0/Nucleic Acids; 0/Proteins; 0/Silicon Compounds; 12033-89-5/silicon nitride; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 82115-62-6/Interferon-gamma; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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