Document Detail

Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.).
MedLine Citation:
PMID:  23143485     Owner:  NLM     Status:  In-Data-Review    
Plant transformation methods are invaluable biotechnological tools to generate specific and targeted genetic variation for performance improvement of crop plants. Genetic information is created by proper modification during gene cloning flanked by proper regulatory sequences and delivered to plants via -different plant transformation techniques. Due to being a multipurpose plant, cotton has been subjected to different genetic transformation methods to provide the breeders with an opportunity to develop alien traits or improve the endogenous gene performance that are very difficult or impossible to develop through conventional breeding methods. Here we describe the novel physical way of cotton transformation with different genes by using embryogeneic calli as continuous source of explants.
Muhammad Arshad; Yusuf Zafar; Shaheen Asad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  958     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-92     Citation Subset:  IM    
Gene Transformation Lab, Agricultural Biotechnology Division (ABD), National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
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