| Silica xerogel as a continuous column support for high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 21619217 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A preliminary study of the chromatographic performance and permeability of a continuous silica xerogel column under reversed-phase HPLC conditions was performed. A porous chromatographic support was synthesized inside a 0.32 mm i.d. × 13 cm fused silica tube from potassium silicate solution and derivatized with dimethyloctadecylchlorosilane. The plate height at 0.01 cm/s (0.5 μL/min), near the apparent optimum linear velocity, was about 65 μm. The column efficiencies in terms of numbers of plates per meter were 5000 and 13 000 for ethyl benzoate (k = 0.8) and naphthalene (k = 2.0), respectively, at 0.5 μL/min. The major parameter affecting column efficiency was the heterogeneous morphology of the xerogel, modifications to which are expected to improve chromatographic performance. The column provided efficiencies comparable to those reported for continuous polymeric columns but less than that previously reported for a continuous silica column. Gradient elution mode was demonstrated with a mixture of polycyclic aromatic hydrocarbons. The column was highly permeable, exhibiting a linear dependence of pressure to flow rate and a back pressure of only 632 psi at 10 μL/min when a 95% aqueous mobile phase was used. |
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Authors:
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S M Fields |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical chemistry Volume: 68 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 2011-05-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2709-12 Citation Subset: - |
Affiliation:
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Hoechst Marion Roussel, Analytical and Structural Sciences, 2110 East Galbraith Road, Cincinnati, Ohio 45215. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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