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Silica-dispersed glucose oxidase for glucose sensing: in vitro testing in serum and blood and the effect of condensation pH.
MedLine Citation:
PMID:  23024461     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objectives of this study were to examine the feasibility of using glucose oxidase (GOx) dispersed in a silica matrix for glucose monitoring in whole blood, and then to assess whether the flexibility of silica sol-gel chemistry could be exploited to enhance glucose sensor performance and stability. Silica-dispersed GOx was deployed on platinized platinum (Pt) wire to form a Clark-type amperometric glucose sensor. Sensors were calibrated using buffered glucose standard solutions, and then tested against glucose spiked human serum and whole blood. All serum and whole blood measurements met the minimum FDA requirement of falling within the "A+B region" of a Clark Error Grid. To our knowledge this is the first report of using silica-dispersed GOx to measure glucose in whole blood. The effect of condensation pH on sensor performance was assessed by dispersing GOx in silica condensed at pH 3, 7 and 12, and then testing the sensor response against glucose calibration standards. The pH 12 silica sensors had statistically faster response time, and higher sensor sensitivity compared to pH 7, pH 3 silica and glutaraldehyde crosslinked sensors. Membranes of the pH 12 silica had statistically higher glucose diffusion coefficient than did the pH 7 and 3 sensors. GOx dispersed in pH 12 silica also had the longest half life. We hypothesize that the gel-like pH 12 silica gels provided reduced barriers to glucose diffusion, and the more aqueous microenvironment provided greater stability for the enzyme.
Authors:
James M Harris; Gabriel P Lopez; William M Reichert
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Sensors and actuators. B, Chemical     Volume:  174     ISSN:  0925-4005     ISO Abbreviation:  Sens Actuators B Chem     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101149755     Medline TA:  Sens Actuators B Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  373-379     Citation Subset:  -    
Affiliation:
Department of Biomedical Engineering Duke University Durham, NC 27708-0281.
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