Document Detail


Silica nanoconstruct cellular toleration threshold in vitro.
MedLine Citation:
PMID:  21342660     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The influence of geometry of silica nanomaterials on cellular uptake and toxicity on epithelial and phagocytic cells was studied. Three types of amine-terminated silica nanomaterials were prepared and characterized via the modified Stober method, namely spheres (178±27 nm), worms (232±22 nm×1348±314 nm) and cylinders (214±29 nm×428±66 nm). The findings of the study suggest that in this size range and for the cell types studied, geometry does not play a dominant role in the modes of toxicity and uptake of these particles. Rather, a concentration threshold and cell type dependent toxicity of all particle types was observed. This correlated with confocal microscopy observations, as all nanomaterials were observed to be taken up in both cell types, with a greater extent in phagocytic cells. It must be noted that there appears to be a concentration threshold at ~100 μg/mL, below which there is limited to no impact of the nanoparticles on membrane integrity, mitochondrial function, phagocytosis or cell death. Analysis of cell morphology by transmission electron microscopy, colocalization experiments with intracellular markers and Western Blot results provide evidence of potential involvement of lysosomal escape, autophagic like activity, compartmental fusion and recycling in response to intracellular nanoparticle accumulation. These processes could be involved in cellular coping or defense mechanisms. The manipulation of physicochemical properties to enhance or reduce toxicity paves the way for the safe design of silica-based nanoparticles for use in nanomedicine.
Authors:
Heather L Herd; Alexander Malugin; Hamidreza Ghandehari
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-02-20
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  153     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-11     Completed Date:  2011-11-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  40-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
Department of Bioengineering, University of Utah, 20 South 2030 East, Salt Lake City, UT 84112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy
Cell Line
Cell Membrane Permeability
Cell Proliferation
Cell Survival
Humans
Lysosomes / metabolism
Mice
Nanostructures / chemistry*,  toxicity*,  ultrastructure
Particle Size
Silicon Dioxide / chemistry,  metabolism*,  toxicity*
Grant Support
ID/Acronym/Agency:
R01 DE019050-05/DE/NIDCR NIH HHS; R01-DE19050/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
7631-86-9/Silicon Dioxide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tumor targeted Quantum Dot-Mucin 1 aptamer-Doxorubicin conjugate for imaging and treatment of cancer...
Next Document:  Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siR...