Document Detail


Silica-iron oxide magnetic nanoparticles modified for gene delivery: a search for optimum and quantitative criteria.
MedLine Citation:
PMID:  22222384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To optimize silica-iron oxide magnetic nanoparticles with surface phosphonate groups decorated with 25-kD branched polyethylenimine (PEI) for gene delivery.
METHODS: Surface composition, charge, colloidal stabilities, associations with adenovirus, magneto-tranduction efficiencies, cell internalizations, in vitro toxicities and MRI relaxivities were tested for the particles decorated with varying amounts of PEI.
RESULTS: Moderate PEI-decoration of MNPs results in charge reversal and destabilization. Analysis of space and time resolved concentration changes during centrifugation clearly revealed that at >5% PEI loading flocculation gradually decreases and sufficient stabilization is achieved at >10%. The association with adenovirus occurred efficiently at levels over 5% PEI, resulting in the complexes stable in 50% FCS at a PEI-to-iron w/w ratio of ≥7%; the maximum magneto-transduction efficiency was achieved at 9-12% PEI. Primary silica iron oxide nanoparticles and those with 11.5% PEI demonstrated excellent r(2)* relaxivity values (>600 s(-1)(mM Fe)(-1)) for the free and cell-internalized particles.
CONCLUSIONS: Surface decoration of the silica-iron oxide nanoparticles with a PEI-to-iron w/w ratio of 10-12% yields stable aqueous suspensions, allows for efficient viral gene delivery and labeled cell detection by MRI.
Authors:
Olga Mykhaylyk; Titus Sobisch; Isabella Almstätter; Yolanda Sanchez-Antequera; Sabine Brandt; Martina Anton; Markus Döblinger; Dietmar Eberbeck; Marcus Settles; Rickmer Braren; Dietmar Lerche; Christian Plank
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-06
Journal Detail:
Title:  Pharmaceutical research     Volume:  29     ISSN:  1573-904X     ISO Abbreviation:  Pharm. Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-09-13     Revised Date:  2012-11-07    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1344-65     Citation Subset:  IM    
Affiliation:
Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, Ismaningerstrasse 22, 81675 Munich, Germany. olga.mykhaylyk@lrz.tu-muenchen.de
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Animals
Cell Line
Colloids / chemistry
Drug Stability
Ferric Compounds / chemistry*
Gene Transfer Techniques*
Genetic Vectors / chemistry*,  genetics
Humans
Lentivirus / genetics
Magnetic Resonance Imaging
Magnetics*
Mice
Microscopy, Electron, Transmission
Models, Molecular
Nanoparticles / chemistry*
Polyethyleneimine / chemistry
Rats
Silicon Dioxide / chemistry*
Surface Properties
X-Ray Diffraction
Chemical
Reg. No./Substance:
0/Colloids; 0/Ferric Compounds; 1309-37-1/ferric oxide; 7631-86-9/Silicon Dioxide; 9002-98-6/Polyethyleneimine
Comments/Corrections
Erratum In:
Pharm Res. 2012 May;29(5):1420

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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