| Silica-iron oxide magnetic nanoparticles modified for gene delivery: a search for optimum and quantitative criteria. | |
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MedLine Citation:
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PMID: 22222384 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To optimize silica-iron oxide magnetic nanoparticles with surface phosphonate groups decorated with 25-kD branched polyethylenimine (PEI) for gene delivery. METHODS: Surface composition, charge, colloidal stabilities, associations with adenovirus, magneto-tranduction efficiencies, cell internalizations, in vitro toxicities and MRI relaxivities were tested for the particles decorated with varying amounts of PEI. RESULTS: Moderate PEI-decoration of MNPs results in charge reversal and destabilization. Analysis of space and time resolved concentration changes during centrifugation clearly revealed that at >5% PEI loading flocculation gradually decreases and sufficient stabilization is achieved at >10%. The association with adenovirus occurred efficiently at levels over 5% PEI, resulting in the complexes stable in 50% FCS at a PEI-to-iron w/w ratio of ≥7%; the maximum magneto-transduction efficiency was achieved at 9-12% PEI. Primary silica iron oxide nanoparticles and those with 11.5% PEI demonstrated excellent r(2)* relaxivity values (>600 s(-1)(mM Fe)(-1)) for the free and cell-internalized particles. CONCLUSIONS: Surface decoration of the silica-iron oxide nanoparticles with a PEI-to-iron w/w ratio of 10-12% yields stable aqueous suspensions, allows for efficient viral gene delivery and labeled cell detection by MRI. |
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Authors:
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Olga Mykhaylyk; Titus Sobisch; Isabella Almstätter; Yolanda Sanchez-Antequera; Sabine Brandt; Martina Anton; Markus Döblinger; Dietmar Eberbeck; Marcus Settles; Rickmer Braren; Dietmar Lerche; Christian Plank |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-01-06 |
Journal Detail:
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Title: Pharmaceutical research Volume: 29 ISSN: 1573-904X ISO Abbreviation: Pharm. Res. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-04-24 Completed Date: 2012-09-13 Revised Date: 2012-11-07 |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: United States |
Other Details:
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Languages: eng Pagination: 1344-65 Citation Subset: IM |
Affiliation:
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Institute of Experimental Oncology and Therapy Research, Klinikum rechts der Isar der Technischen Universität München, Ismaningerstrasse 22, 81675 Munich, Germany. olga.mykhaylyk@lrz.tu-muenchen.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Cell Line Colloids / chemistry Drug Stability Ferric Compounds / chemistry* Gene Transfer Techniques* Genetic Vectors / chemistry*, genetics Humans Lentivirus / genetics Magnetic Resonance Imaging Magnetics* Mice Microscopy, Electron, Transmission Models, Molecular Nanoparticles / chemistry* Polyethyleneimine / chemistry Rats Silicon Dioxide / chemistry* Surface Properties X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Colloids; 0/Ferric Compounds; 1309-37-1/ferric oxide; 7631-86-9/Silicon Dioxide; 9002-98-6/Polyethyleneimine |
| Comments/Corrections | |
Erratum In:
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Pharm Res. 2012 May;29(5):1420 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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