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Silibinin induces protective superoxide generation in human breast cancer MCF-7 cells.
MedLine Citation:
PMID:  19968587     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The pharmacological activity of polyphenolic silibinin from milk thistle (Silybum marianum) is primarily due to its antioxidant property. However, this study found that silibinin promoted sustained superoxide (O(2)(.-)) production that was specifically scavenged by exogenous superoxide dismutase (SOD) in MCF-7 cells, while the activity of endogenous SOD was not changed by silibinin. Previous work proved that silibinin induced MCF-7 cell apoptosis through mitochondrial pathway and this study further proved that O(2)(.-) generation induced by silibinin was also related to mitochondria. It was found that respiratory chain complexes I, II and III were all involved in silibinin-induced O(2)(.-) generation. Moreover, it was found that silibinin-induced O(2)(.-) had protective effect, as exogenous SOD markedly enhanced silibinin-induced apoptosis.
Authors:
Hong-Jun Wang; Yuan-Yuan Jiang; Xiao-Feng Wei; Huai Huang; Shin-Ichi Tashiro; Satoshi Onodera; Takashi Ikejima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Free radical research     Volume:  44     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  90-100     Citation Subset:  IM    
Affiliation:
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
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