Document Detail


Silibinin attenuates oxidative metabolism and cytokine production by monocytes from preeclamptic women.
MedLine Citation:
PMID:  23316804     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Silibinin is a polyphenolic plant flavonoid with anti-inflammatory properties. The present study investigated the effect of silibinin on oxidative metabolism and cytokine production - tumor necrosis factor-alpha (TNF-α), interleukin (IL)12, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-6, IL-10, and transforming growth factor beta (TGF-β(1)) - by peripheral blood monocytes (PBM) from preeclamptic pregnant women. It is a case controlled study involving women with preeclampsia (PE, n=30) compared with normotensive pregnant (NT, n=30) and with non-pregnant (NP, n=30) women. Monocytes were obtained and cultured with or without silibinin (5 μM or 50 μM) for 18h. Superoxide anion (O(2)-) and hydrogen peroxide (H(2)O(2)) release were determined by specific assays, and cytokine levels were determined by immunoenzymatic assays (ELISA). Monocytes from preeclamptic women cultured without stimulus released higher levels of O(2)-, H(2)O(2) and TNF-α, and lower levels of IL-10 and TGF-β(1) than did monocytes from NT and NP women. Treatment in vitro with silibinin significantly inhibited spontaneous O(2)- and H(2)O(2) release and TNF-α production by monocytes from preeclamptic women. The main effect of silibinin was obtained at 50 μM concentration. Thus, silibinin exerts anti-oxidative and anti-inflammatory effects on monocytes from preeclamptic pregnant women by inhibiting the in vitro endogenous release of reactive oxygen species and TNF-α production.
Authors:
Renata Cristofalo; Camila F Bannwart-Castro; Claudia G Magalhães; Vera T M Borges; Jose C Peraçoli; Steven S Witkin; Maria T Peraçoli
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-15
Journal Detail:
Title:  Free radical research     Volume:  -     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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