Document Detail

Silencing of proviruses in embryonic cells: efficiency, stability and chromatin modifications.
MedLine Citation:
PMID:  23154467     Owner:  NLM     Status:  MEDLINE    
Embryonic stem cells repress retroviral infection through transcriptional silencing of proviral DNAs. We characterized two distinct mechanisms of silencing in embryonic mouse cells infected by Moloney murine leukaemia virus (MLV): a highly efficient one targeting the proline transfer RNA primer-binding site (PBSpro), and a less efficient one operating independently of the PBS. Rare virus-expressing populations were isolated, and the timing and efficiency of establishment of silencing were determined. Superinfection of the selected virus-expressing cells with a second virus carrying a distinguishable reporter revealed that the PBSpro-directed silencing was still largely intact, whereas the PBS-independent silencing was partially reduced. The timing and stability of silencing, and the associated chromatin modifications on newly established and endogenous proviruses were determined. The results indicate that epigenetic mechanisms with different specificity and efficiency are used to silence the exogenous retroviral sequences in embryonic cells.
Sharon Schlesinger; Stephen P Goff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-16
Journal Detail:
Title:  EMBO reports     Volume:  14     ISSN:  1469-3178     ISO Abbreviation:  EMBO Rep.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-06-03     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  100963049     Medline TA:  EMBO Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  73-9     Citation Subset:  IM    
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MeSH Terms
Chromatin / genetics,  metabolism
DNA Methylation
DNA, Viral / antagonists & inhibitors,  genetics*
Embryonic Stem Cells / cytology,  metabolism*,  virology*
Epigenesis, Genetic*
Gene Silencing
Genes, Reporter
Green Fluorescent Proteins
Host-Pathogen Interactions
Moloney murine leukemia virus / genetics*
NIH 3T3 Cells
Proviruses / genetics*
RNA, Small Interfering / genetics
RNA, Transfer, Pro / genetics,  metabolism
Signal Transduction
Grant Support
R37 CA 30488/CA/NCI NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Chromatin; 0/DNA, Viral; 0/RNA, Small Interfering; 0/RNA, Transfer, Pro; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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