Document Detail

Silencing or amplification of endocannabinoid signaling in blastocysts via CB1 compromises trophoblast cell migration.
MedLine Citation:
PMID:  22833670     Owner:  NLM     Status:  MEDLINE    
Endocannabinoid signaling plays key roles in multiple female reproductive events. Previous studies have shown an interesting phenomenon, that mice with either silenced or elevated endocannabinoid signaling via Cnr1 encoding CB(1) show similar defects in several pregnancy events, including preimplantation embryo development. To unravel the downstream signaling of this phenomenon, microarray studies were performed using RNAs collected from WT, Cnr1(-/-), and Faah(-/-) mouse blastocysts on day 4 of pregnancy. The results indicate that about 100 genes show unidirectional changes under either silenced or elevated anandamide signaling via CB(1). Functional enrichment analysis of the microarray data predicted that multiple biological functions and pathways are affected under aberrant endocannabinoid signaling. Among them, genes enriched in cell migration are suppressed in Cnr1(-/-) or Faah(-/-) blastocysts. Cell migration assays validated the prediction of functional enrichment analysis that cell mobility and spreading of either Cnr1(-/-) or Faah(-/-) trophoblast stem cells are compromised. Either silenced or elevated endocannabinoid signaling via CB(1) causes similar changes in downstream targets in preimplantation embryos and trophoblast stem cells. This study provides evidence that a tightly regulated endocannabinoid signaling is critical to normal preimplantation embryo development and migration of trophoblast stem cells.
Huirong Xie; Xiaofei Sun; Yulan Piao; Anil G Jegga; Stuart Handwerger; Minoru S H Ko; Sudhansu K Dey
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2012-07-24
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-17     Completed Date:  2012-12-04     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  32288-97     Citation Subset:  IM    
Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio 45229, USA.
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MeSH Terms
Blastocyst / cytology
Cell Movement
Embryo Implantation
Endocannabinoids / metabolism*
Gene Silencing*
Mice, Inbred C57BL
Mice, Transgenic
Oligonucleotide Array Sequence Analysis
Receptor, Cannabinoid, CB1 / metabolism*
Signal Transduction
Stem Cells / cytology
Trophoblasts / metabolism*
Wound Healing
Grant Support
Reg. No./Substance:
0/Endocannabinoids; 0/Receptor, Cannabinoid, CB1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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