| Silencing or amplification of endocannabinoid signaling in blastocysts via CB1 compromises trophoblast cell migration. | |
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MedLine Citation:
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PMID: 22833670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endocannabinoid signaling plays key roles in multiple female reproductive events. Previous studies have shown an interesting phenomenon, that mice with either silenced or elevated endocannabinoid signaling via Cnr1 encoding CB(1) show similar defects in several pregnancy events, including preimplantation embryo development. To unravel the downstream signaling of this phenomenon, microarray studies were performed using RNAs collected from WT, Cnr1(-/-), and Faah(-/-) mouse blastocysts on day 4 of pregnancy. The results indicate that about 100 genes show unidirectional changes under either silenced or elevated anandamide signaling via CB(1). Functional enrichment analysis of the microarray data predicted that multiple biological functions and pathways are affected under aberrant endocannabinoid signaling. Among them, genes enriched in cell migration are suppressed in Cnr1(-/-) or Faah(-/-) blastocysts. Cell migration assays validated the prediction of functional enrichment analysis that cell mobility and spreading of either Cnr1(-/-) or Faah(-/-) trophoblast stem cells are compromised. Either silenced or elevated endocannabinoid signaling via CB(1) causes similar changes in downstream targets in preimplantation embryos and trophoblast stem cells. This study provides evidence that a tightly regulated endocannabinoid signaling is critical to normal preimplantation embryo development and migration of trophoblast stem cells. |
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Authors:
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Huirong Xie; Xiaofei Sun; Yulan Piao; Anil G Jegga; Stuart Handwerger; Minoru S H Ko; Sudhansu K Dey |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't Date: 2012-07-24 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-17 Completed Date: 2012-12-04 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 32288-97 Citation Subset: IM |
Affiliation:
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Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio 45229, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology Cell Movement Embryo Implantation Endocannabinoids / metabolism* Female Gene Silencing* Male Mice Mice, Inbred C57BL Mice, Transgenic Oligonucleotide Array Sequence Analysis Receptor, Cannabinoid, CB1 / metabolism* Signal Transduction Stem Cells / cytology Trophoblasts / metabolism* Wound Healing |
| Grant Support | |
ID/Acronym/Agency:
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DA006668/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Endocannabinoids; 0/Receptor, Cannabinoid, CB1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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