| Silencing of Ref-1 expression by retrovirus-mediated shRNA sensitizes HEK293 cells to hydrogen peroxide-induced apoptosis. | |
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MedLine Citation:
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PMID: 19060414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Redox factor-1 (Ref-1) is a multifunctional protein involved in DNA base excision repair (BER) and transcription factor modification. By the use of retrovirus-delivered shRNA, we effectively suppressed endogenous Ref-1 expression in human embryonic kidney (HEK) 293 cells. Our results showed that downregulation of Ref-1 rendered cells more sensitive to H(2)O(2)-induced apoptosis. In this process, the absence of Ref-1 decreased the ratio of Bcl-2/Bax protein expression, which resulted in cytochrome c release and caspase-3 activation. These data indicate that constitutive Ref-1 is required for cellular defense and that this protective function is dependent on its role in the regulation of Bcl-2 family proteins. |
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Authors:
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Jian-Jun Xiong; Yan Zhang; Jia-Li Wang; Guo-Dong Bao; Yang Zhang; Zhen-Yu Zhu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 72 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-24 Completed Date: 2009-02-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 3206-10 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Sun Yat-sen University, Guangzhou, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects*,
genetics Base Sequence Caspase 3 / metabolism Cell Line Cytochromes c / metabolism DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics*, metabolism Enzyme Activation Gene Silencing* Humans Hydrogen Peroxide / toxicity* Oxidative Stress / genetics Proto-Oncogene Proteins c-bcl-2 / genetics RNA, Small Interfering / genetics, metabolism* Retroviridae / genetics* bcl-2-Associated X Protein / genetics |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Small Interfering; 0/bcl-2-Associated X Protein; 7722-84-1/Hydrogen Peroxide; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC 4.2.99.18/APEX1 protein, human; EC 4.2.99.18/DNA-(Apurinic or Apyrimidinic Site) Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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