Document Detail

Silencing of Ref-1 expression by retrovirus-mediated shRNA sensitizes HEK293 cells to hydrogen peroxide-induced apoptosis.
MedLine Citation:
PMID:  19060414     Owner:  NLM     Status:  MEDLINE    
Redox factor-1 (Ref-1) is a multifunctional protein involved in DNA base excision repair (BER) and transcription factor modification. By the use of retrovirus-delivered shRNA, we effectively suppressed endogenous Ref-1 expression in human embryonic kidney (HEK) 293 cells. Our results showed that downregulation of Ref-1 rendered cells more sensitive to H(2)O(2)-induced apoptosis. In this process, the absence of Ref-1 decreased the ratio of Bcl-2/Bax protein expression, which resulted in cytochrome c release and caspase-3 activation. These data indicate that constitutive Ref-1 is required for cellular defense and that this protective function is dependent on its role in the regulation of Bcl-2 family proteins.
Jian-Jun Xiong; Yan Zhang; Jia-Li Wang; Guo-Dong Bao; Yang Zhang; Zhen-Yu Zhu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  72     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-02-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  3206-10     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Sun Yat-sen University, Guangzhou, China.
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MeSH Terms
Apoptosis / drug effects*,  genetics
Base Sequence
Caspase 3 / metabolism
Cell Line
Cytochromes c / metabolism
DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics*,  metabolism
Enzyme Activation
Gene Silencing*
Hydrogen Peroxide / toxicity*
Oxidative Stress / genetics
Proto-Oncogene Proteins c-bcl-2 / genetics
RNA, Small Interfering / genetics,  metabolism*
Retroviridae / genetics*
bcl-2-Associated X Protein / genetics
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Small Interfering; 0/bcl-2-Associated X Protein; 7722-84-1/Hydrogen Peroxide; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3; EC protein, human; EC or Apyrimidinic Site) Lyase

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