| Silencing HIF-1α reduces the adhesion and secretion functions of acute leukemia hBMSCs. | |
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MedLine Citation:
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PMID: 22948410 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system was constructed by putting the two cells into the same suitable cultured media and conditions. Cell adhesion and secretion functions of stromal cells were evaluated after transfection with the lentiviral RNAi vector of HIF-1α. Increased HIF-1α mRNA and protein was detected in the nucleus of the acute myeloblastic and acute lymphoblastic leukemia compared with normal BMSCs. The lentiviral RANi vector for HIF-1α was successfully constructed and was applied to block the expression of HIF-1α. When HIF-1α of BMSCs was blocked, the expression of VEGF and SDF-1 secreted by stromal cells were decreased. When HIF-1α was blocked, the co-cultured Jurkat cell's adhesion and migration functions were also decreased. Taken together, these results suggest that HIF-1α acts as an important transcription factor and can significantly affect the secretion and adhesion functions of leukemia BMSCs. |
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Authors:
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Zeng Dong-Feng; Liu Ting; Chang Cheng; Zhang Xi; Liang Xue; Chen Xing-Hua; Kong Pei-Yan |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] Volume: 45 ISSN: 1414-431X ISO Abbreviation: Braz. J. Med. Biol. Res. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8112917 Medline TA: Braz J Med Biol Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 906-12 Citation Subset: IM |
Affiliation:
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Department of Hematology, XinQiao Hospital, Third Military Medical University, ChongQing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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