Document Detail

Silencing of Connexin 43 Suppresses Invasion, Migration and Lung Metastasis of Rat Hepatocellular Carcinoma Cells.
MedLine Citation:
PMID:  22320152     Owner:  NLM     Status:  Publisher    
To reduce cancer mortality, understanding of mechanisms of cancer metastasis is crucial. We have established 6 rat hepatocellular carcinoma (HCC) cell lines which exhibit differing metastatic potential to the lung after inoculation into the tail veins of nude mice. In the present experiment, we investigated the process of cell attachment to metastatic sites and possible regulating factors. One hour after inoculation, 2 of 2 HCC cell lines with high metastatic potential and 1 of 2 HCC cell lines with low metastatic potential exhibited many attached cells in the lung. One day after inoculation, lung metastatic foci were observed only with highly-metastatic cells with elevated connexin 43 (Cx43) expression as assessed by cDNA array analysis. Furthermore, 24 or 48 hrs after transfection of an siRNA targeting Cx43, in vitro invasion and migration were suppressed by 68% (P<0.001) and 36% (P<0.05) compared with control-siRNA transfected cells, despite no differences in cellular morphology, cell proliferation or apoptotic activity. Moreover, the number of metastatic nodules per lung area in nude mice was significantly (P<0.01) reduced. In conclusion, suppression of Cx43 expression in tumor cells reduced in vitro migration and invasion capacity and in vivo metastatic ability so that Cx43 has potential as a molecular target for prevention of cancer metastasis with Cx43 overexpressing tumors. © 2012 Japanese Cancer Association.
Kumiko Ogawa; Pornsiri Pitchakarn; Shugo Suzuki; Teera Chewonarin; Mingxi Tang; Seishiro Takahashi; Aya Naiki-Ito; Shinya Sato; Satoru Takahashi; Makoto Asamoto; Tomoyuki Shirai
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-9
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Japanese Cancer Association.
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan; Division of Pathology, National Institute of Health Sciences, Tokyo, Japan.
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