Document Detail


Signs of apoptosis in the pituitary, thyroid and ovaries of female rats after a single dose of potassium iodide.
MedLine Citation:
PMID:  20799850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim of this work was to study the effect of a single dose of iodide on the expression of apoptosis mediating enzymes (caspase 32 and caspase 8) as well as of antiapoptopic protein Bcl-2 in the anterior pituitary, thyroid and ovaries in rats.
METHODS: A total of 24 inbred rat females of local laboratory strain weighing 250-300 g at the stage of diestrus and/or metestrus were used. Pituitary, thyroid and ovaries were dissected from the first group (8 control rats) at the same time as these from the second group (8 rats) and third group (8 rats) which were given potassium iodide (KI; 4 microg/100g body weight by gavage) 48 hours and 120 hours prior to the sacrifice, respectively. Streptavidin-biotin method was used to define the expression of caspase 8 and 32 as well as that of Bcl-2 with the aid of specific monoclonal antibodies in terms of the percentage of positive immunostained area (% PA).
RESULTS: Histological examinations of endocrine glands did not show any signs of necrosis. In the thyroid a significant decrease of both caspases 32 and 8 was found at 120 hrs after KI administration. In contrast, however, in the pituitary a significant increase of caspase 32 appeared at 120 hrs after KI administration, although a considerable, but not significant increase of both caspases 32 and 8 already appeared after 48 hrs. In the ovaries a considerable and about the same increase of both caspases 32 and 8 appeared at 120 hrs after KI, though the difference was significant only for caspase 8. In addition, however, a considerable, but not significant, increase of caspase 32 at 48 hrs also appeared in the ovaries.
CONCLUSION: The apoptotic activity was induced in the pituitary and ovaries of female rats by a single dose of KI, as shown by considerable increase of both the effectory caspase 32 and initiatory caspase 8 at 48 and 120 hrs after iodide administration. In contrast, however, in the thyroid any considerable changes in caspase 32 and caspase 8 were not observed after 48 hrs, while a significant decrease of both appeared after 120 hrs. Although no changes in Bcl-2 were found in the pituitary and thyroid, considerable and significant decrease appeared in the ovaries after 120 hrs.
Authors:
N Basalaeva; G Sychugov; N Miphtakhutdinov; V Strizhikov
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrine regulations     Volume:  44     ISSN:  1210-0668     ISO Abbreviation:  Endocr Regul     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2011-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9112018     Medline TA:  Endocr Regul     Country:  Slovakia    
Other Details:
Languages:  eng     Pagination:  83-8     Citation Subset:  IM    
Affiliation:
Medical Provision at South Ural Railways, The Territorial Branch of Russian Railways State-Owned Joint-Stock Company, Chelyabinsk, Russia. nadezhda.basalaeva@gmail.com
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Caspase 8 / biosynthesis
Caspases / biosynthesis
Female
Ovary / drug effects,  pathology
Pituitary Gland, Anterior / drug effects,  pathology
Potassium Iodide / pharmacology*
Rats
Thyroid Gland / drug effects,  pathology
bcl-Associated Death Protein / biosynthesis
Chemical
Reg. No./Substance:
0/Bad protein, rat; 0/bcl-Associated Death Protein; 7681-11-0/Potassium Iodide; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases; EC 3.4.22.-/caspase 32, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Importance of pulsing illumination parameters in low-level-light therapy.
Next Document:  Fos expression in tyrosine hydroxylase containing hypothalamic neurons in CRH-KO mice: effect of imm...