Document Detail


Significant blood resistance to nitric oxide transfer in the lung.
MedLine Citation:
PMID:  20150569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lung diffusing capacity for nitric oxide (DLNO) is used to measure alveolar membrane conductance (DMNO), but disagreement remains as to whether DMNO=DLNO, and whether blood conductance (thetaNO)=infinity. Our previous in vitro and in vivo studies suggested that thetaNO<infinity. We now show in a membrane oxygenator model perfused with whole blood that addition of a cell-free bovine hemoglobin (Hb) glutamer-200 solution increased diffusing capacity of the circuit (D) for NO (DNO) by 39%, D for carbon monoxide (DCO) by 24%, and the ratio of DNO to DCO by 12% (all P<0.001). In three anesthetized dogs, DLNO and DLCO were measured by a rebreathing technique before and after three successive equal volume-exchange transfusions with bovine Hb glutamer-200 (10 ml/kg each, total exchange 30 ml/kg). At baseline, DLNO/DLCO=4.5. After exchange transfusion, DLNO rose 57+/-16% (mean+/-SD, P=0.02) and DLNO/DLCO=7.1, whereas DLCO remained unchanged. Thus, in vitro and in vivo data directly demonstrate a finite thetaNO. We conclude that the erythrocyte and/or its immediate environment imposes considerable resistance to alveolar-capillary NO uptake. DLNO is sensitive to dynamic hematological factors and is not a pure index of conductance of the alveolar tissue membrane. With successive exchange transfusion, the estimated in vivo thetaNO [5.1 ml NO.(ml blood.min.Torr)(-1)] approached 4.5 ml NO.(ml blood.min.Torr)(-1), which was derived from in vitro measurements by Carlsen and Comroe (J Gen Physiol 42: 83-107, 1958). Therefore, we suggest use of thetaNO=4.5 ml NO.(min.Torr.ml blood)(-1) for calculation of DM(NO) and pulmonary capillary blood volume from DLNO and DLCO.
Authors:
Colin D R Borland; Helen Dunningham; Fiona Bottrill; Alain Vuylsteke; Cuneyt Yilmaz; D Merrill Dane; Connie C W Hsia
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-02-11
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  108     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-08-12     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1052-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Substitutes / pharmacology
Blood Volume
Capillary Permeability* / drug effects
Carbon Monoxide / blood
Cell Membrane Permeability* / drug effects
Dogs
Erythrocytes / drug effects,  metabolism*
Exchange Transfusion, Whole Blood
Hemoglobins / pharmacology
Hemolysis
Lung / blood supply*,  drug effects,  metabolism*
Male
Models, Cardiovascular
Nitric Oxide / blood*
Pulmonary Circulation*
Pulmonary Diffusing Capacity* / drug effects
Time Factors
Grant Support
ID/Acronym/Agency:
HL-054060/HL/NHLBI NIH HHS; HL-062873/HL/NHLBI NIH HHS; R01 HL-040070/HL/NHLBI NIH HHS; R01 HL040070/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/hemoglobin glutamer-200; 31C4KY9ESH/Nitric Oxide; 7U1EE4V452/Carbon Monoxide
Comments/Corrections
Comment In:
J Appl Physiol (1985). 2010 May;108(5):1027-9   [PMID:  20224003 ]

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