| Significant blood resistance to nitric oxide transfer in the lung. | |
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MedLine Citation:
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PMID: 20150569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lung diffusing capacity for nitric oxide (DLNO) is used to measure alveolar membrane conductance (DMNO), but disagreement remains as to whether DMNO=DLNO, and whether blood conductance (thetaNO)=infinity. Our previous in vitro and in vivo studies suggested that thetaNO<infinity. We now show in a membrane oxygenator model perfused with whole blood that addition of a cell-free bovine hemoglobin (Hb) glutamer-200 solution increased diffusing capacity of the circuit (D) for NO (DNO) by 39%, D for carbon monoxide (DCO) by 24%, and the ratio of DNO to DCO by 12% (all P<0.001). In three anesthetized dogs, DLNO and DLCO were measured by a rebreathing technique before and after three successive equal volume-exchange transfusions with bovine Hb glutamer-200 (10 ml/kg each, total exchange 30 ml/kg). At baseline, DLNO/DLCO=4.5. After exchange transfusion, DLNO rose 57+/-16% (mean+/-SD, P=0.02) and DLNO/DLCO=7.1, whereas DLCO remained unchanged. Thus, in vitro and in vivo data directly demonstrate a finite thetaNO. We conclude that the erythrocyte and/or its immediate environment imposes considerable resistance to alveolar-capillary NO uptake. DLNO is sensitive to dynamic hematological factors and is not a pure index of conductance of the alveolar tissue membrane. With successive exchange transfusion, the estimated in vivo thetaNO [5.1 ml NO.(ml blood.min.Torr)(-1)] approached 4.5 ml NO.(ml blood.min.Torr)(-1), which was derived from in vitro measurements by Carlsen and Comroe (J Gen Physiol 42: 83-107, 1958). Therefore, we suggest use of thetaNO=4.5 ml NO.(min.Torr.ml blood)(-1) for calculation of DM(NO) and pulmonary capillary blood volume from DLNO and DLCO. |
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Authors:
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Colin D R Borland; Helen Dunningham; Fiona Bottrill; Alain Vuylsteke; Cuneyt Yilmaz; D Merrill Dane; Connie C W Hsia |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-02-11 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 108 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-05 Completed Date: 2010-08-12 Revised Date: 2011-10-19 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1052-60 Citation Subset: IM |
Affiliation:
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Department of Medicine, Hinchingbrooke Hospital, Huntingdon PE29 6NT, UK. colin.borland@hinchingbrooke.nhs.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Substitutes / pharmacology Blood Volume Capillary Permeability* / drug effects Carbon Monoxide / blood Cell Membrane Permeability* / drug effects Dogs Erythrocytes / drug effects, metabolism* Exchange Transfusion, Whole Blood Hemoglobins / pharmacology Hemolysis Lung / blood supply*, drug effects, metabolism* Male Models, Cardiovascular Nitric Oxide / blood* Pulmonary Circulation* Pulmonary Diffusing Capacity* / drug effects Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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HL-054060/HL/NHLBI NIH HHS; HL-062873/HL/NHLBI NIH HHS; R01 HL-040070/HL/NHLBI NIH HHS; R01 HL040070-23/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Substitutes; 0/Hemoglobins; 0/hemoglobin glutamer-200; 10102-43-9/Nitric Oxide; 630-08-0/Carbon Monoxide |
| Comments/Corrections | |
Comment In:
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J Appl Physiol. 2010 May;108(5):1027-9
[PMID:
20224003
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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