Document Detail

Significant reduction of ATP production in PHA-activated CD4+ cells in 1-day-old blood from transplant patients.
MedLine Citation:
PMID:  23135604     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Global immunosuppression can be measured by assessing adenosine triphospate (ATP) levels in mitogen-stimulated CD4+ T cells.
METHODS: We investigated the effect of storage time on ATP levels in 234 blood samples from 18 healthy individuals and 152 transplant patients. The difference between day 0 (<13 hours post-blood draw) and day 1 (24-37 hours) measurements was analyzed and compared with various factors; a subset of samples was also analyzed in 6-hour intervals.
RESULTS: The ATP levels were significantly lower on day 1 compared with that on day 0 in healthy individuals (279±159 vs 414±159 ng/mL, P<0.001) and patients (356±209 vs 455±221 ng/mL, P<0.0001). Of the 18 healthy individuals, 17 showed ATP reduction, whereas 192 (89%) of 216 patients did so on day 1 (24.8±24.1%). In the time course analysis, ATP levels decreased with the blood storage time in healthy and patient samples, and the reduction began as early as 7 hours post-blood draw. The reduction rate was significantly higher in patient samples with low day 0 ATP levels compared with samples with moderate or high levels (44.7±31.3% vs 23.2±23.6% or 18.7±15.7%; P<0.001). The reduction rate in patients treated with alemtuzumab induction was slightly higher than that in daclizumab-treated patients (28.8±24.6% vs 21.3±21.3%, P=0.09). CD4+ cell number did not change within 24 hours post-blood draw, but CD4 expression decreased 2.0±2.8% (P<0.05).
CONCLUSIONS: The ATP levels are significantly lower in 1-day-old blood compared with fresh blood, suggesting that fresh blood should be used for assessing the T cell immune function to obtain the most accurate results.
Elina Suviolahti; Anna Petrosyan; James Mirocha; Shili Ge; Artur Karasyov; David Thomas; Odette Galera; Washington Lim; Anne Maria Jimenez; Lawrence S C Czer; George Chaux; Jeffrey De Leon; Andy Pao; Stanley C Jordan; Mieko Toyoda
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Transplantation     Volume:  94     ISSN:  1534-6080     ISO Abbreviation:  Transplantation     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  2013-02-21     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1243-9     Citation Subset:  IM    
Transplant Immunology Laboratory, Comprehensive Transplant Center, Cedars-Sinai Medical Center/UCLA School of Medicine, Los Angeles, CA 90048, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism*
Antibodies, Monoclonal, Humanized / administration & dosage
Antineoplastic Agents / administration & dosage
Blood Specimen Collection / standards
CD4-Positive T-Lymphocytes / drug effects,  metabolism*
Immune Tolerance / immunology*
Immunoglobulin G / administration & dosage
Immunologic Techniques / standards*
Immunosuppressive Agents / administration & dosage*
Lymphocyte Count
Organ Transplantation*
Phytohemagglutinins / diagnostic use*,  pharmacology
Reproducibility of Results
Time Factors
Young Adult
Reg. No./Substance:
0/Antibodies, Monoclonal, Humanized; 0/Antineoplastic Agents; 0/Immunoglobulin G; 0/Immunosuppressive Agents; 0/Phytohemagglutinins; 3A189DH42V/alemtuzumab; 56-65-5/Adenosine Triphosphate; CUJ2MVI71Y/daclizumab

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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