Document Detail

Significant IgG-immunoreactivity of the spermatogonia of the germ cell-depleted testis after busulfan treatment.
MedLine Citation:
PMID:  15941629     Owner:  NLM     Status:  MEDLINE    
Busulfan kills spermatogonia with the exception of a few that are attached to the basal membrane of the seminiferous epithelium. In mice, these remaining spermatogonia reacted strongly to a goat anti-mouse IgG antibody. Spermatogonia in untreated testes rarely showed the same reactivity. Testicular IgG levels are normally minimal but increase markedly, 4 weeks after busulfan treatment before peaking at week 6. Laser scanning cytometry analysis of control and busulfan-treated testicular cells showed busulfan treatment increased the frequency of cells that were positive for not only IgG (from 0.67+/-0.29 to 16.5+/-3.8%) but also for alpha6-integrin, beta1-integrin, GFR(-1 and/or Ret. Thus, an enrichment in putative male stem cells correlates with appearance of IgG expression. Confocal microscopy revealed busulfan-treated cells contained both IgG and GFRalpha-1, and that the initial surface IgG became intracellular in the weeks following busulfan treatment. The basement membranes of the seminiferous tubules were compromised by busulfan treatment as the mRNA expression profiles of various adhesion molecules in the basement membranes were altered and electron microscopy revealed severe damage. Serum IgG levels increased in a manner corresponding with the increase in testicular IgG levels. Thus, it appears that in the busulfan-treated testis, small breaches of the blood-testis barrier leak IgG that is then taken up by a significant number of spermatogonia. When the busulfan-resistant germ cells were transferred into recipient germ cell-depleted testes, they settled and repopulated the recipient testes. Thus, the IgG-bearing cells observed after busulfan treatment may be putative spermatogonial stem cells.
Yun-Jung Choi; Hyuk Song; Deug-Nam Kwon; Seong-Keun Cho; Sung-Jo Kang; Sung-Moon Yoe; Heung-Cheol Kim; Hoon-Taek Lee; Chankyu Park; Jin-Hoi Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-06
Journal Detail:
Title:  Animal reproduction science     Volume:  91     ISSN:  0378-4320     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2005-11-25     Completed Date:  2006-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  317-35     Citation Subset:  IM    
Department of Dairy Science, Division of Animal Science and Technology, College of Agriculture and Life Science, Gyeongsang National University, Jinju, GyeongNam 660-701, Republic of Korea.
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MeSH Terms
Alkylating Agents / pharmacology*
Antibodies, Anti-Idiotypic / immunology
Antigens, CD29
Busulfan / pharmacology*
Cell Death / drug effects
Immunoglobulin G / immunology*
Integrin alpha6
Mice, Inbred ICR
Microscopy, Electron
RNA / analysis
Reverse Transcriptase Polymerase Chain Reaction
Spermatogonia / immunology*,  transplantation
Spermatozoa / chemistry,  drug effects*,  transplantation
Testis / chemistry,  cytology*,  drug effects
Reg. No./Substance:
0/Alkylating Agents; 0/Antibodies, Anti-Idiotypic; 0/Antigens, CD29; 0/Immunoglobulin G; 0/Integrin alpha6; 55-98-1/Busulfan; 63231-63-0/RNA

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