| Significant expression of interleukin 15 in rat experimental severe acute pancreatitis. | |
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MedLine Citation:
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PMID: 20332642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: In severe acute pancreatitis (SAP), multiple organ dysfunction syndrome is a contributor to high mortality. We recently demonstrated that the serum interleukin (IL)-15 level is a predictor of the complications and mortality in clinical SAP. The aim was to investigate the role of IL-15 in experimental SAP. MATERIALS AND METHODS: SAP was induced by retrograde injection of 3 and 20% sodium deoxycholate (DCA) into biliopancreatic ducts in rats (DCA pancreatitis). Expressions of IL-15 were evaluated by Western blotting and immunohistochemical staining. Recombinant IL-15 protein was administered intraperitoneally, and the effects were investigated. RESULTS: Western blotting revealed the expressions of IL-15 in the pancreas, liver, lung and intestine in 3% DCA pancreatitis. Immunohistochemical staining showed the expression of IL-15 in the cytoplasm of each organ. In 3% DCA pancreatitis, administration of recombinant IL-15 protein attenuated the elevation of serum alanine aminotransferase (ALT) levels and improved the morphological change of the lung 18 h after the induction of SAP. Moreover, in 20% DCA pancreatitis, IL-15 improved the elevation of serum amylase and ALT levels 6 h after the induction. CONCLUSIONS: These results suggest that IL-15 is related to organ dysfunction during SAP, and that IL-15 functions as a protective factor against the organ injuries. |
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Authors:
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K Kamei; T Yasuda; T Ueda; F Qiang; H Shiozaki; H Ohyanagi; Y Takeyama |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-24 |
Journal Detail:
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Title: European surgical research. Europäische chirurgische Forschung. Recherches chirurgicales européennes Volume: 44 ISSN: 1421-9921 ISO Abbreviation: Eur Surg Res Publication Date: 2010 |
Date Detail:
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Created Date: 2010-07-29 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0174752 Medline TA: Eur Surg Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 159-69 Citation Subset: IM |
Copyright Information:
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(c) 2010 S. Karger AG, Basel. |
Affiliation:
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Department of Surgery, Kinki University School of Medicine, Osaka-Sayama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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blood Amylases / blood Animals Deoxycholic Acid / toxicity Humans Immunohistochemistry Interleukin-15 / metabolism*, therapeutic use Intestine, Small / drug effects, microbiology, pathology Lung Injury / drug therapy, pathology Male Pancreatitis / chemically induced, drug therapy, immunology*, metabolism Rats Rats, Wistar Recombinant Proteins / therapeutic use Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-15; 0/Recombinant Proteins; 83-44-3/Deoxycholic Acid; EC 2.6.1.2/Alanine Transaminase; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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