Document Detail


Significance and problems of histopathological examination and utility of real-time reverse transcriptase-polymerase chain reaction method for the detection of sentinel lymph node metastasis in breast cancer.
MedLine Citation:
PMID:  17986798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sentinel lymph node (SLN) biopsy has become a common procedure for breast cancer patients. Many reported feasibility studies have revealed a high identification (95.4% of 2,125 cases) and accuracy rate on histopathological analysis (94% frozen and 97% paraffin). However, about 5 to 8% of cases false-negative are in this examination. Various methods of histopathological examination have been reported by many authors, but the Philadelphia consensus meeting recommended that sections less than 2.0 mm in thickness should be prepared and examined to detect metastasis larger than 2.0 mm. In addition, immunohistochemistry for cytokeratin is considered to be useful to detect isolated tumor cells (ITC) in the representative sections. On the other hand, real-time reverse transcriptase-polymerase chain reaction (RT-PCR) might detect micrometastasis (larger than 0.2 mm) (sensitivity and specificity; 89.5% and 96.7% frozen sections) and expected to be used as a routine method instead of histopathological examination.
Authors:
Masafumi Kurosumi; Hiroyuki Takei
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Breast cancer (Tokyo, Japan)     Volume:  14     ISSN:  1880-4233     ISO Abbreviation:  Breast Cancer     Publication Date:  2007  
Date Detail:
Created Date:  2007-11-07     Completed Date:  2008-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888201     Medline TA:  Breast Cancer     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  342-9     Citation Subset:  IM    
Affiliation:
Department of Pathology Saitama Cancer Center, Saitama 362-0806, Japan. mkurosumi@cancer-c.pref.saitama.jp
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / pathology*
Cytodiagnosis
Diagnosis, Differential
Humans
Immunoenzyme Techniques
Lymph Nodes / pathology*
Lymphatic Metastasis / diagnosis
Neoplasm Staging
Predictive Value of Tests
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sentinel Lymph Node Biopsy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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