| Significance of cell proliferation markers (Minichromosome maintenance protein 7, topoisomerase IIalpha and Ki-67) in cavital fluid cytology: can we differentiate reactive mesothelial cells from malignant cells? | |
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MedLine Citation:
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PMID: 19821496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to evaluate whether immunocytochemical expressions of proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), topoisomerase IIalpha (topo IIalpha), and Ki-67, in reactive mesothelial cells and malignant cells obtained from cavital fluids could be useful for their differential diagnosis. Samples diagnosed as reactive mesothelial cells (14 cases) or malignant tumors (28 cases) in cavital fluids were examined. Immunocytochemical staining of MCM 7, topo IIalpha, and Ki-67 was performed with the universal immunoperoxidase polymer method. In reactive mesothelial cells, MCM 7 was stained in a fine granular pattern and its distribution was uniform in the nuclei. Topo IIalpha and Ki-67 were stained in a coarse granular pattern and the distributions were the same as MCM 7. In contrast, in malignant cells, MCM 7 was stained in an irregular and fine granular pattern, and topo IIalpha and Ki-67 were stained in a uniform and coarse granular pattern. Labeling indices of MCM 7 (cut-off value; 30%, sensitivity; 100%, and specificity; 100%), topo IIalpha (cut-off value; 15%, sensitivity; 89.3%, and specificity; 92.9%) and Ki-67 (cut-off value; 30%, sensitivity; 64.3%, and specificity; 92.9%) of malignant cells were significantly higher than those of reactive mesothelial cells. MCM 7, topo IIalpha, and Ki-67 are different types of cell proliferation markers. MCM 7 and topo IIalpha, in particular, could be reliable tools for differential diagnosis between reactive mesothelial cells and malignant cells. |
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Authors:
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Fumikazu Kimura; Jumpei Kawamura; Jun Watanabe; Shingo Kamoshida; Kenji Kawai; Isao Okayasu; Sadahito Kuwao |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Diagnostic cytopathology Volume: 38 ISSN: 1097-0339 ISO Abbreviation: Diagn. Cytopathol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-10 Completed Date: 2010-04-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506895 Medline TA: Diagn Cytopathol Country: United States |
Other Details:
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Languages: eng Pagination: 161-7 Citation Subset: IM |
Affiliation:
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Division of Diagnostic Pathology and Cytology, Higashiyamato Hospital, Tokyo, Japan. k1100rs@yamatokai.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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metabolism,
pathology Aged Antigens, Neoplasm / metabolism* Ascitic Fluid / metabolism, pathology* Carcinoma, Squamous Cell / metabolism, pathology Cell Cycle Proteins / metabolism* Cell Proliferation* DNA Topoisomerases, Type II, Eukaryotic / metabolism* DNA-Binding Proteins / metabolism* Epithelium / metabolism, pathology Female Humans Immunoenzyme Techniques Ki-67 Antigen / metabolism* Male Mesothelioma / metabolism, pathology Nuclear Proteins / metabolism* Peritoneal Neoplasms / metabolism, pathology Pleural Neoplasms / metabolism, pathology Small Cell Lung Carcinoma / metabolism, pathology Thoracic Cavity / metabolism, pathology* Tumor Markers, Biological / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Ki-67 Antigen; 0/MCM7 protein, human; 0/Nuclear Proteins; 0/Tumor Markers, Biological; EC 5.99.1.-/DNA Topoisomerases, Type II, Eukaryotic; EC 5.99.1.3/DNA topoisomerase II alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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