Document Detail

Significance of the 56th tyrosine on cytotoxic activity of tumor necrosis factor.
MedLine Citation:
PMID:  1457718     Owner:  NLM     Status:  MEDLINE    
After using site-specific mutagenesis a conserved amino acid residue, the 56th tyrosine in human tumor necrosis was substituted by glutamine. The expression level of the mutant protein in E. coli did not changed significantly, however, its specific activity of the cytotoxicity on L929 cultural cells was decreased to 1/583 of that of the unmutant tumor necrosis factor. The results show that the 56th tyrosine residue or the region where tyrosine 56th located is important for the cytotoxic activity of tumor necrosis factor.
D Zhang; S Chen; N Chen; G Zhi; C Su
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese journal of biotechnology     Volume:  8     ISSN:  1042-749X     ISO Abbreviation:  Chin. J. Biotechnol.     Publication Date:  1992  
Date Detail:
Created Date:  1993-01-12     Completed Date:  1993-01-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9100855     Medline TA:  Chin J Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23-8     Citation Subset:  IM    
Fourth Military Medical University, Xian, China.
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MeSH Terms
Base Sequence
Cytotoxicity, Immunologic / genetics*
Gene Expression Regulation / physiology
Molecular Sequence Data
Mutagenesis, Site-Directed
Oligonucleotides / chemical synthesis
Structure-Activity Relationship
Tumor Necrosis Factor-alpha / genetics*,  immunology
Tyrosine / genetics*
Reg. No./Substance:
0/Oligonucleotides; 0/Tumor Necrosis Factor-alpha; 55520-40-6/Tyrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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