| Signaling via GPR30 protects the myocardium from ischemia/reperfusion injury. | |
| | |
MedLine Citation:
|
PMID: 20434187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Estrogen may protect against the development of cardiovascular disease. Recently, a receptor known as GPR30 that seems to mediate estrogen's nongenomic effects has been identified. We hypothesized that the activation of GPR30 protects cardiac function and decreases myocardial inflammation after global ischemia/reperfusion (I/R). METHODS: Hearts from male Sprague-Dawley rats were perfused via Langendorff and treated with either (1) vehicle; (2) 10 nm of the GPR30 agonist, G-1; or (3) 100 nm of G-1; they then were subjected to 25 minutes of ischemia and 40 minutes of reperfusion. Cardiac functional parameters were measured continuously. Ventricular tissue was analyzed for tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6. RESULTS: At end-reperfusion, the left ventricular developed pressure in the 100-nm G-1 group was improved compared with vehicle (26% +/- 12% equilibrium vs 54% +/- 9% equilibrium; P < .05). Similar findings were noted when comparing the 100-nm G-1 group with the vehicle in terms of +dP/dt (53% +/- 12% equilibrium vs 26% +/- 19%, respectively; P < .05) and -dP/dt (56% +/- 15% equilibrium vs 22% +/- 16% equilibrium, respectively; P < .05). TNF-alpha, IL-1beta, and IL-6 levels were lower in myocardium of the 100-nm G-1 group compared with the vehicle (P < .05). CONCLUSION: The GPR30 agonist, G-1, improves functional recovery and decreases myocardial inflammation after global I/R. GPR30 may play an important role in estrogen's ability to protect the heart against I/R injury. |
| | |
Authors:
|
Brent R Weil; Mariuxi C Manukyan; Jeremy L Herrmann; Yue Wang; Aaron M Abarbanell; Jeffrey A Poynter; Daniel R Meldrum |
Related Documents
:
|
16650907 - Intracoronary aqueous oxygen perfusion, performed 24 h after the onset of postinfarctio... 9228647 - Role of eicosanoid inhibition of ischemia reperfusion injury: intact and isolated rat h... 11893897 - Effects of sm-20550, a new na(+)/h(+) exchange inhibitor, on survival and infarct size ... 19997057 - Activation of peroxisome proliferator-activated receptor-beta/delta attenuates myocardi... 2021717 - Swift trial of delayed elective intervention v conservative treatment after thrombolysi... 20224387 - Risk stratification of ventricular arrhythmias in patients with systolic heart failure. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Surgery Volume: 148 ISSN: 1532-7361 ISO Abbreviation: Surgery Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-16 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417347 Medline TA: Surgery Country: United States |
Other Details:
|
Languages: eng Pagination: 436-43 Citation Subset: AIM; IM |
Copyright Information:
|
Copyright 2010 Mosby, Inc. All rights reserved. |
Affiliation:
|
Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cyclopentanes / pharmacology Estrogens / metabolism Heart / drug effects, physiopathology Inflammation Mediators / metabolism Interleukin-1beta / metabolism Interleukin-6 / metabolism Male Myocardial Reperfusion Injury / drug therapy, metabolism*, physiopathology, prevention & control* Myocardium / metabolism* Quinolines / pharmacology Rats Rats, Sprague-Dawley Receptors, G-Protein-Coupled / agonists, metabolism* Signal Transduction Tumor Necrosis Factor-alpha / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
F32HL092718/HL/NHLBI NIH HHS; F32HL092719/HL/NHLBI NIH HHS; F32HL093987/HL/NHLBI NIH HHS; R01GM070628/GM/NIGMS NIH HHS; R01HL085595/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; 0/Cyclopentanes; 0/Estrogens; 0/GPR30 protein, rat; 0/Inflammation Mediators; 0/Interleukin-1beta; 0/Interleukin-6; 0/Quinolines; 0/Receptors, G-Protein-Coupled; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clostridium perfringens epsilon toxin inhibits the gastrointestinal transit in mice.
Next Document: Establishment of lentiviral-vector-mediated model of human alpha-1 antitrypsin delivery into hepatoc...