Document Detail


Signaling alkaline pH stress in the yeast Saccharomyces cerevisiae through the Wsc1 cell surface sensor and the Slt2 MAPK pathway.
MedLine Citation:
PMID:  17088254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alkalinization of the external environment represents a stress situation for Saccharomyces cerevisiae. Adaptation to this circumstance involves the activation of diverse response mechanisms, the components of which are still largely unknown. We show here that mutation of members of the cell integrity Pkc1/Slt2 MAPK module, as well as upstream and downstream elements of the system, confers sensitivity to alkali. Alkalinization resulted in fast and transient activation of the Slt2 MAPK, which depended on the integrity of the kinase module and was largely abolished by sorbitol. Lack of Wsc1, removal of specific extracellular and intracellular domains, or substitution of Tyr(303) in this putative membrane stress sensor rendered cells sensitive to alkali and considerably decreased alkali-induced Slt2 activation. In contrast, constitutive activation of Slt2 by the bck1-20 allele increased pH tolerance in the wsc1 mutant. DNA microarray analysis revealed that several genes encoding cell wall proteins, such as GSC2/FKS2, DFG5, SKT5, and CRH1, were induced, at least in part, by high pH in an Slt2-dependent manner. We observed that dfg5, skt5, and particularly dfg5 skt5 cells were alkali-sensitive. Therefore, our results show that an alkaline environment imposes a stress condition on the yeast cell wall. We propose that the Slt2-mediated MAPK pathway plays an important role in the adaptive response to this insult and that Wsc1 participates as an essential cell-surface pH sensor. Moreover, these results provide a new example of the complexity of the response of budding yeast to the alkalinization of the environment.
Authors:
Raquel Serrano; Humberto Martín; Antonio Casamayor; Joaquín Ariño
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-06
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  281     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-25     Completed Date:  2007-02-12     Revised Date:  2012-06-08    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39785-95     Citation Subset:  IM    
Affiliation:
Departament de Bioquímica i Biología Molecular, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological / genetics
Cell Wall / enzymology,  genetics,  physiology
Enzyme Activation / genetics
Hydrogen-Ion Concentration
MAP Kinase Signaling System / physiology*
Membrane Proteins / genetics,  physiology*
Mitogen-Activated Protein Kinase Kinases / genetics
Mitogen-Activated Protein Kinases / physiology*
Mutagenesis, Site-Directed
Receptors, Cell Surface / physiology*
Saccharomyces cerevisiae / enzymology,  genetics,  physiology*
Saccharomyces cerevisiae Proteins / genetics,  physiology*
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Receptors, Cell Surface; 0/SLG1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.11.1/BCK1 protein, S cerevisiae; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/SLT2 protein, S cerevisiae; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

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