Document Detail

The signaling adaptor Eps8 is an essential actin capping protein for dendritic cell migration.
MedLine Citation:
PMID:  21835647     Owner:  NLM     Status:  MEDLINE    
Dendritic cells (DCs) flexibly adapt to different microenvironments by using diverse migration strategies that are ultimately dependent on the dynamics and structural organization of the actin cytoskeleton. Here, we have shown that DCs require the actin capping activity of the signaling adaptor Eps8 to polarize and to form elongated migratory protrusions. DCs from Eps8-deficient mice are impaired in directional and chemotactic migration in 3D in vitro and are delayed in reaching the draining lymph node (DLN) in vivo after inflammatory challenge. Hence, Eps8-deficient mice are unable to mount a contact hypersensitivity response. We have also shown that the DC migratory defect is cell autonomous and that Eps8 is required for the proper architectural organization of the actin meshwork and dynamics of cell protrusions. Yet, Eps8 is not necessary for antigen uptake, processing, and presentation. Thus, we have identified Eps8 as a unique actin capping protein specifically required for DC migration.
Emanuela Frittoli; Gianluca Matteoli; Andrea Palamidessi; Elisa Mazzini; Luigi Maddaluno; Andrea Disanza; Changsong Yang; Tatyana Svitkina; Maria Rescigno; Giorgio Scita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-11
Journal Detail:
Title:  Immunity     Volume:  35     ISSN:  1097-4180     ISO Abbreviation:  Immunity     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-29     Completed Date:  2011-11-15     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  9432918     Medline TA:  Immunity     Country:  United States    
Other Details:
Languages:  eng     Pagination:  388-99     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
IFOM, FIRC Institute of Molecular Oncology Foundation at IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy.
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MeSH Terms
Actin Capping Proteins / immunology*
Adaptor Proteins, Signal Transducing / deficiency,  genetics,  immunology*
Antigen Presentation
Cell Movement / immunology
Cell Proliferation
Cells, Cultured
Cytoskeletal Proteins / deficiency,  genetics,  immunology*
Dendritic Cells / immunology*
Dermatitis, Contact / immunology
Flow Cytometry
Mice, Inbred C57BL
Mice, Knockout
Signal Transduction*
T-Lymphocytes / immunology
Grant Support
Reg. No./Substance:
0/Abi1 protein, mouse; 0/Actin Capping Proteins; 0/Adaptor Proteins, Signal Transducing; 0/Cytoskeletal Proteins

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